Sulfo-NHS-LC-Biotin sodium

Name: Sulfo-NHS-LC-Biotin sodium
Brand: MedChemExpress
SKU: HY-D0799
Rating: 4.5 (1 reviews)

Cat. No.: HY-D0799 Purity: 98.32%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Sulfo-NHS-LC-Biotin sodium is an amine-reactive biotinylation reagent that can be used for antibody labeling.

For research use only. We do not sell to patients.

Sulfo-NHS-LC-Biotin sodium Chemical Structure

CAS No. : 191671-46-2

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 132	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 132	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 77	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Sulfo-NHS-LC-Biotin sodium

•Ecotoxicol Environ Saf. 2022 Aug 31;244:114046. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Sulfo-NHS-LC-Biotin sodium is an amine-reactive biotinylation reagent that can be used for antibody labeling^[1].

Molecular Weight

556.59

Formula

C₂₀H₂₉N₄NaO₉S₂

CAS No.

191671-46-2

Appearance

Solid

Color

White to yellow

SMILES

O=C(C(CC1=O)S(=O)(O[Na])=O)N1OC(CCCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (149.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (35.93 mM; ultrasonic and warming and adjust pH to 9 with NaOH)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7967 mL	8.9833 mL	17.9665 mL
5 mM	0.3593 mL	1.7967 mL	3.5933 mL
10 mM	0.1797 mL	0.8983 mL	1.7967 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.32%

Select Batch:

Data Sheet (269 KB) SDS (419 KB)

COA (245 KB) HNMR (387 KB) LCMS (89 KB)

Handling Instructions (2659 KB)

References

[1]. K Hoya, et al. A Novel Intravascular Drug Delivery Method Using Endothelial Biotinylation and Avidin-Biotin Binding. Drug Deliv. Oct-Dec 2001;8(4):215-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7967 mL	8.9833 mL	17.9665 mL	44.9164 mL
	5 mM	0.3593 mL	1.7967 mL	3.5933 mL	8.9833 mL
	10 mM	0.1797 mL	0.8983 mL	1.7967 mL	4.4916 mL
	15 mM	0.1198 mL	0.5989 mL	1.1978 mL	2.9944 mL
	20 mM	0.0898 mL	0.4492 mL	0.8983 mL	2.2458 mL
	25 mM	0.0719 mL	0.3593 mL	0.7187 mL	1.7967 mL
	30 mM	0.0599 mL	0.2994 mL	0.5989 mL	1.4972 mL
DMSO	40 mM	0.0449 mL	0.2246 mL	0.4492 mL	1.1229 mL
	50 mM	0.0359 mL	0.1797 mL	0.3593 mL	0.8983 mL
	60 mM	0.0299 mL	0.1497 mL	0.2994 mL	0.7486 mL
	80 mM	0.0225 mL	0.1123 mL	0.2246 mL	0.5615 mL
	100 mM	0.0180 mL	0.0898 mL	0.1797 mL	0.4492 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.