Sulfo-NHS-LC-Biotin
Based on 9 publication(s) in Google Scholar
Sulfo-NHS-LC-Biotin is an amine-reactive ester derivative of biotin and cannot pass the intact blood-retinal barrier. It can be used to assess vascular permeability of the brain and retinal vasculature.
For research use only. We do not sell to patients.
- CAS No.: 190598-57-3
- Formula: C20H30N4O9S2
- Molecular Weight:534.60
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Sulfo-NHS-LC-Biotin
More- Cell Metab. 2025 Oct 7;37(10):1998-2013.e7. [Abstract]
- Cell Host Microbe. 2026 Mar 11;34(3):406-424.e10. [Abstract]
- J Extracell Vesicles. 2024 Jul;13(7):e12494. [Abstract]
- Adv Sci (Weinh). 2025 Dec 14:e02250. [Abstract]
- Cell Death Discov. 2026 May 20;12(1):300. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Jun 16;123(24):e2524671123. [Abstract]
- Ecotoxicol Environ Saf. 2025 Mar 1:292:117962. [Abstract]
- Ecotoxicol Environ Saf. 2022 Oct 1:244:114046. [Abstract]
- Mol Genet Genomics. 2024 May 24;299(1):57. [Abstract]
Customer Validation & Images
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Gel Electrophoresis
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Gel Electrophoresis
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Histological Imaging/Staining
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WB
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Flow Cytometry
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Calculations:
For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.
1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:
mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.
* 20 = Recommended molar fold excess of biotin for 2 mg/mL protein sample
2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction:
mmoL Biotin × (1000000 μL/L) × (L/10 mmoL) =μL Biotin
Biotin Labeling Reaction:
1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations.
2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO.
3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution.
4. PBS or other amine-free buffer having pH 7-8 for use as reaction buffer.
5. Incubate reaction on ice for two hours or at room temperature for 30 minutes.
6. To tests the labeled protein by ELISA or Western blot.
6. Wash cells three times with ice-cold PBS (pH 8.0) to remove amine-containing media and proteins from the cells.
7. Suspend cells at a concentration of ~25 × 106 cells/ml in PBS (pH 8.0).
8. Add 1.0 mg of Sulfo-NHS-LC-Biotin reagent per mL of cell suspension (results in ~2 mM biotin reagent).
9. Incubate reaction mixture at room temperature for 30 minutes.
Note: Performing this incubation at 4°C may reduce active internalization of the biotin reagent.
10. Wash cells three times with PBS + 100 mM Glycine to quench and remove excess biotin reagent and byproducts.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 190598-57-3
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Molecular Weight 534.60
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Formula C20H30N4O9S2
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SMILES
O=C(C(CC1=O)S(=O)(O)=O)N1OC(CCCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (9)
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Journal Impact Factor
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Most Recent
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Cell Metab
Catenibacteriummitsuokai promotes hepatocellular carcinogenesis by binding to hepatocytes and generating quinolinic acid. [Abstract]2025 Oct 7;37(10):1998-2013.e7. PMID: 41005296
Sulfo-NHS-LC-Biotin purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Oct 7;37(10):1998-2013.e7. [Abstract]
Sulfo-NHS-LC-Biotin sodium (1 mM; 2-4 h; 4 ℃) was used for far western screening of C mitsuokai adhesins using mass spectrometry.
Sulfo-NHS-LC-Biotin purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Oct 7;37(10):1998-2013.e7. [Abstract]
Sulfo-NHS-LC-Biotin sodium (1 mM; 2-4 h; 4 ℃). The process and screening of C mitsuokai adhesins by coomassie blue staining in combination with mass spectrometry after Far Western assay in Huh7.
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Cell Host Microbe
Intratumoral bacterium Enterocloster bolteae promotes hepatocellular carcinoma progression by directly binding tumor cells. [Abstract]2026 Mar 11;34(3):406-424.e10. PMID: 41742409 -
J Extracell Vesicles
RAB22A sorts epithelial growth factor receptor (EGFR) from early endosomes to recycling endosomes for microvesicles release. [Abstract]2024 Jul;13(7):e12494. PMID: 39051763
Sulfo-NHS-LC-Biotin purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Jul;13(7):e12494. [Abstract]
Sulfo-NHS-LC-Biotin sodium (0.08 mg/mL; 30 min). Representative Western blot showed cell surface EGFR levels on HeLa cells.
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Adv Sci (Weinh)
Circular RNA PTPN4 Contributes to Blood-Brain Barrier Disruption during Early Epileptogenesis. [Abstract]2025 Dec 14:e02250. PMID: 41391036 -
Cell Death Discov
FBXW7α regulates amyloid pathology by mediating ubiquitination and degradation of BACE1 in Alzheimer's disease. [Abstract]2026 May 20;12(1):300. PMID: 42161904 -
Proc Natl Acad Sci U S A
Nf2 orchestrates β-arrestin2-biased PTH1R signaling to couple bone mass with skeletal integrity. [Abstract]2026 Jun 16;123(24):e2524671123. PMID: 42268882 -
Ecotoxicol Environ Saf
Emerging per- and polyfluoroalkyl substance perfluoro-(3,5,7,9-tetraoxadecanoic) acid (PFO4DA) impairs steroidogenesis and spermatogenesis by suppressing StAR and CYP11A1 expression in mice. [Abstract]2025 Mar 1:292:117962. PMID: 40009946
Sulfo-NHS-LC-Biotin purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Mar 1:292:117962. [Abstract]
Sulfo-NHS-LC-Biotin sodium (50 μL; 30 min). Biotin tracer signals showed BTB (blood-testis barrier) permeability in BALB/c mice followed PFO4DA treatment.
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Ecotoxicol Environ Saf
Lead suppresses interferon γ to induce splenomegaly via modification on splenic endothelial cells and lymphoid tissue organizer cells in mice. [Abstract]2022 Oct 1:244:114046. PMID: 36057201
Sulfo-NHS-LC-Biotin purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2022 Oct 1:244:114046. [Abstract]
Sulfo-NHS-LC-Biotin sodium (0.2 mg/mL; 37℃; 30 min). Representative flow plots for the biotin+ RBC in the blood of recipient mice for the RBC clearance assay.
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Mol Genet Genomics
Precise diagnosis of a hereditary spherocytosis patient with complicated hematological phenotype. [Abstract]2024 May 24;299(1):57. PMID: 38787432
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sulfo-NHS-LC-Biotin Related Classifications
HY-D0799A Related Classifications
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