Biotin-LC-LC-NHS

Cat. No.: HY-W040254 Purity: ≥95.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Biotin-LC-LC-NHS is a SMCC cross-linking reagent that can be used to mark antibody and other small molecules, such as Paclitaxel.

For research use only. We do not sell to patients.

Biotin-LC-LC-NHS Chemical Structure

CAS No. : 89889-52-1

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Purity & Documentation
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Description

Biotin-LC-LC-NHS is a SMCC cross-linking reagent that can be used to mark antibody and other small molecules, such as Paclitaxel^[1]^[2].

In Vitro

Biotinylation of antibodies is accomplished by the reaction of Biotin-LC-LC-NHS (1 mg/mL in dimethyl formamide) with the protein in aqueous phase pH 7.4. The molar ratio of biotin to antibody in the labeling reaction was 10:1 (the final concentration of biotin was 70 μM) ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

567.70

Formula

C₂₆H₄₁N₅O₇S

CAS No.

89889-52-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NCCCCCC(NCCCCCC(ON1C(CCC1=O)=O)=O)=O)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (220.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7615 mL	8.8075 mL	17.6149 mL
5 mM	0.3523 mL	1.7615 mL	3.5230 mL
10 mM	0.1761 mL	0.8807 mL	1.7615 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (269 KB) SDS (252 KB)

COA (242 KB) HNMR (229 KB)

Handling Instructions (2659 KB)

References

[1]. K Dill, et al. Immunoassays Based on Electrochemical Detection Using Microelectrode Arrays. Biosens Bioelectron. 2004 Nov 1;20(4):736-42. [Content Brief]

[2]. Lev G Lis, et al. Synthesis and Biological Evaluation of a Biotinylated Paclitaxel With an Extra-Long Chain Spacer Arm. ACS Med Chem Lett . 2012 Sep 13;3(9):745-748. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7615 mL	8.8075 mL	17.6149 mL	44.0373 mL
	5 mM	0.3523 mL	1.7615 mL	3.5230 mL	8.8075 mL
	10 mM	0.1761 mL	0.8807 mL	1.7615 mL	4.4037 mL
	15 mM	0.1174 mL	0.5872 mL	1.1743 mL	2.9358 mL
	20 mM	0.0881 mL	0.4404 mL	0.8807 mL	2.2019 mL
	25 mM	0.0705 mL	0.3523 mL	0.7046 mL	1.7615 mL
	30 mM	0.0587 mL	0.2936 mL	0.5872 mL	1.4679 mL
	40 mM	0.0440 mL	0.2202 mL	0.4404 mL	1.1009 mL
	50 mM	0.0352 mL	0.1761 mL	0.3523 mL	0.8807 mL
	60 mM	0.0294 mL	0.1468 mL	0.2936 mL	0.7340 mL
	80 mM	0.0220 mL	0.1101 mL	0.2202 mL	0.5505 mL
	100 mM	0.0176 mL	0.0881 mL	0.1761 mL	0.4404 mL

Biotin-LC-LC-NHS Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Biotin-LC-LC-NHS89889-52-1Fluorescent DyeInhibitorinhibitorinhibit

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