Biotin-LC-LC-NHS
Based on 1 Customer Validation
Biotin-LC-LC-NHS is a biotinylation reagent that contains an NHS moiety, which can react efficiently with primary amino (-NH2). Biotin-LC-LC-NHS enables simple and efficient biotinylation of antibodies, proteins, and any other primary amine-containing biomolecules in solution. Biotin-LC-LC-NHS can be used to biotinylate small molecules such as Paclitaxel (HY-B0015) for use in binding site characterization.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 89889-52-1
- Formula: C26H41N5O7S
- Molecular Weight:567.70
-
Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Biotinylation of antibodies is accomplished by the reaction of Biotin-LC-LC-NHS (1 mg/mL in DMSO) with the protein in aqueous phase pH 7.4. The molar ratio of biotin to antibody in the labeling reaction was 10:1 (the final concentration of biotin was 70 μM) [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 89889-52-1
-
Appearance Solid
-
Molecular Weight 567.70
-
Formula C26H41N5O7S
-
Color White to light yellow
-
SMILES
O=C(NCCCCCC(NCCCCCC(ON1C(CCC1=O)=O)=O)=O)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (176.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (268 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. K Dill, et al. Immunoassays Based on Electrochemical Detection Using Microelectrode Arrays. Biosens Bioelectron. 2004 Nov 1;20(4):736-42. [Content Brief]
[2]. Lev G Lis, et al. Synthesis and Biological Evaluation of a Biotinylated Paclitaxel With an Extra-Long Chain Spacer Arm. ACS Med Chem Lett . 2012 Sep 13;3(9):745-748. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7615 mL | 8.8075 mL | 17.6149 mL | 44.0373 mL |
| 5 mM | 0.3523 mL | 1.7615 mL | 3.5230 mL | 8.8075 mL | |
| 10 mM | 0.1761 mL | 0.8807 mL | 1.7615 mL | 4.4037 mL | |
| 15 mM | 0.1174 mL | 0.5872 mL | 1.1743 mL | 2.9358 mL | |
| 20 mM | 0.0881 mL | 0.4404 mL | 0.8807 mL | 2.2019 mL | |
| 25 mM | 0.0705 mL | 0.3523 mL | 0.7046 mL | 1.7615 mL | |
| 30 mM | 0.0587 mL | 0.2936 mL | 0.5872 mL | 1.4679 mL | |
| 40 mM | 0.0440 mL | 0.2202 mL | 0.4404 mL | 1.1009 mL | |
| 50 mM | 0.0352 mL | 0.1761 mL | 0.3523 mL | 0.8807 mL | |
| 60 mM | 0.0294 mL | 0.1468 mL | 0.2936 mL | 0.7340 mL | |
| 80 mM | 0.0220 mL | 0.1101 mL | 0.2202 mL | 0.5505 mL | |
| 100 mM | 0.0176 mL | 0.0881 mL | 0.1761 mL | 0.4404 mL |
Biotin-LC-LC-NHS Related Classifications
HY-W040254 Related Classifications
Powered by Bioz