Phthalides from Pittosporum illicioides var. illicioides with inhibitory activity on superoxide generation and elastase release by neutrophils

  • J Nat Prod. 2008 Oct;71(10):1692-5. doi: 10.1021/np8004503.
Tsung-Hsien Chou  1 ,  Ih-Sheng Chen ,  Tsong-Long Hwang ,  Tai-Chi Wang ,  Tzong-Huei Lee ,  Lin-Yang Cheng ,  Ya-Chih Chang ,  Jui-Ying Cho ,  Jih-Jung Chen
Affiliations
  • 1. Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China.
PMID: 18817445 DOI: 10.1021/np8004503
Abstract

Six new phthalides, (S)-3-ethyl-7-hydroxy-6-methoxyphthalide (1), (S)-3-ethyl-7-hydroxy-5,6-dimethoxyphthalide (2), (S)-3-ethyl-5,6,7-trimethoxyphthalide (3), (R)-3-ethyl-7-hydroxy-6-methoxyphthalide (4), (Z)-3-ethylidene-7-hydroxy-6-methoxyphthalide (5), and (Z)-3-ethylidene-6,7-dimethoxyphthalide (6), have been isolated from the root of Pittosporum illicioides var. illicioides, together with seven known compounds. The structures of these new compounds were determined through spectroscopic and MS analyses. Compounds 1-4 exhibited inhibition (IC50<or=29.8 microM) of superoxide anion generation by human neutrophils in response to formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B (fMLP/CB). Compounds 5 and 6 inhibited fMLP/CB-induced Elastase release with IC50 values of 38.6+/-4.3 and 33.9+/-3.9 microM, respectively.