Protein Purification

Protein Purification refers to the process of isolating a target protein from complex biological samples (such as cell lysates, tissue homogenates, culture supernatants, etc.) while removing other impurities including proteins, nucleic acids, and lipids. The ultimate goal is to obtain the target protein with high purity, high activity, and high concentration for subsequent research (e.g., structural analysis, functional assays, antibody production, drug development, etc.).
Comprehensive Product Range – To meet different protein purification needs, we offer a wide selection of tags, including Flag (DYKDDDDK), HA, GST, 6X His, GFP, Myc, and more.
Diverse Product Options – Depending on your experimental requirements, we provide magnetic beads, agarose magnetic beads, agarose gel, dry powder chromatography media, and chromatography columns, supporting purification scales ranging from micrograms (μg) to low kilograms (kg).
High Performance – High binding capacity, low background, and strong specificity.
Cost Efficiency – High cost-effectiveness with customizable specifications.

Magnetic Stand (1)

Cat. No.: HY-K0200A Magnetic Stand Plus

MCE Magnetic Stand Plus features an upgraded design that is compatible with multiple centrifuge tube formats, including 200 μL, 1.5 mL, 2 mL, 5 mL, 10 mL, and 15 mL tubes. It adopts a detachable strong magnetic block design, allowing flexible operation for samples of different volumes and improving both convenience and separation efficiency during experiments.

Magnetic Beads (20)

Cat. No.: HY-K0202 Protein A/G Magnetic Beads

MCE Protein A/G Magnetic Beads provide a fast and convenient method for Immunoprecipitation, Co-Immunoprecipitation and Chromatin Immunoprecipitation. The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0201 Anti-HA Magnetic Beads

MCE Anti-HA Magnetic Beads are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0206 Anti-c-Myc Magnetic Beads

MCE Anti-c-Myc Magnetic Beads are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0208 Streptavidin Magnetic Beads

MCE Streptavidin Magnetic Beads provide a fast and convenient method for numerous applications, including purification of proteins and nucleic acids, protein interaction studies, immunoprecipitation, immunoassays, pull-down and cell isolation. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0204 Protein G Magnetic Beads

Protein G Magnetic Beads provide a fast and convenient method for Immunoprecipitation and Co-Immunoprecipitation and Chromatin Immunoprecipitation. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0203 Protein A Magnetic Beads

Protein A Magnetic Beads provide a fast and convenient method for Immunoprecipitation and Co-Immunoprecipitation and Chromatin Immunoprecipitation.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0207 Anti-Flag Magnetic Beads

MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0205 Protein L Magnetic Beads

Protein L Magnetic Beads provide a fast and convenient method for Immunuoprecipitaion, Co-Immunoprecipitation and Chromatin Immunoprecipitation. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0222 Anti-GST Magnetic Beads

MCE Anti-GST Magnetic Beads are used for immunoprecipitation (IP) of specific GST-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0209 Anti-His Magnetic Beads

MCE Anti-His Magnetic Beads are used for immunoprecipitation (IP) of specific His-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0201A Anti-HA Magnetic Beads (1 μm)

MCE Anti-HA Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells andin vitro expression systems. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0224 Amino Magnetic Beads (200 nm, 10 mg/mL)

MCE Amino magnetic beads (200 nm，10 mg/mL) can easily and efficiently combine with a variety of biological ligand in high loads, such as proteins, peptides, oligonucleotides, drug molecules, etc. It can be used as a good basic material for subsequent processing, adsorption, chemical modification and other follow-up processing.

Cat. No.: HY-K0225 Carboxyl Magnetic Beads (200 nm, 10 mg/mL)

MCE Carboxyl Magnetic beads (200 nm, 10 mg/mL) are characterized by superparamagnetism, fast magnetic response, abundant carboxyl functional groups, monodispersity, and submicron scale particle size. Biological ligands (proteins, peptides, oligonucleotides, drug molecules, etc.) can be covalently coupled to the surface of microspheres under the action of special chemical reagents (such as EDC).

Cat. No.: HY-K0228 Oligo (dT)30 Magnetic Beads

MCE Oligo (dT)30 Magnetic Beads are designed for the rapid isolation of highly purified, intact mRNA from eukaryotic total RNA or directly from crude extracts of cells, plant and animal tissues. The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0223 Hydroxyl Magnetic Beads (200 nm, 10 mg/mL)

MCE Hydroxyl Magnetic beads (200 nm, 10 mg/mL) can rapidly isolate nucleic acids from biological samples, which is conducive to the automation and high throughput extraction of nucleic acids.

Cat. No.: HY-K0227 NHS Magnetic Beads (200nm, 10 mg/mL)

MCE NHS Magnetic Beads (200 nm, 10 mg/mL) contain N-hydroxysuccinimide (NHS) functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

Cat. No.: HY-K0226 CHO Magnetic Beads (200 nm, 10 mg/mL)

MCE CHO Magnetic Beads (200 nm, 10 mg/mL) contain CHO functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

Cat. No.: HY-K0247 ConA Magnetic Beads

MCE ConA Magnetic Beads can be used to isolate cells or purify glycoproteins from serum and cell extracts. It is also employed in experiments such as collecting and immobilizing cell nuclei, CUT & Run, and CUT & Tag. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0249 Anti-V5 Magnetic Beads

MCE Anti-V5 Magnetic Beads are well suited for the detection and purification of V5-tagged fusion proteins, as well as for applications such as immunoprecipitation (IP) and co-immunoprecipitation (Co-IP). The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K0250 Anti-MBP Magnetic Beads

Anti-MBP Magnetic Beads are suitable for the detection and purification of MBP-tagged fusion proteins, as well as for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) applications. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Affinity Chromatography Column (1)

Cat. No.: HY-K0221 Affinity Chromatography Column

MCE Affinity Chromatography (AC) Columns are designed for purification of recombinant proteins with different tags, enzymes, antibodies, antigens and nucleic acids.

Agarose Beads (36)

Cat. No.: HY-K0210 Ni-NTA His-Tag Purification Agarose

MCE Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose reagent, enables high-yield, high-purity purification of his-tagged proteins.

Cat. No.: HY-K0211 Glutathione Agarose

MCE Glutathione Agarose, a 4% highly cross-linked agarose reagent, enables high-yield, high-purity purification of GST-tagged proteins.

Cat. No.: HY-K0217 Anti-Flag Affinity Gel

MCE Anti-Flag Affinity Gel is used for immunoprecipitation (IP) or protein purification of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems.

Cat. No.: HY-K0213 Protein A Agarose

MCE Protein A Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein A, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG, IgE, IgM.

Cat. No.: HY-K0214 Protein G Agarose

MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

Cat. No.: HY-K0215 Protein L Agarose

MCE Protein L Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein L, effectively purifies mammalian monoclonal and polyclonal antibodies which have kappa light chain.

Cat. No.: HY-K0219 High-Affinity Iodoacetyl Agarose

MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification.

Cat. No.: HY-K0220 Ni-NTA 6FF Prepacked Column

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

Cat. No.: HY-K0218A Streptavidin Agarose 6FF

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

Cat. No.: HY-K0230 Protein A/G Agarose

MCE Protein A/G Agarose is an affinity chromatography medium for separation and purification of immunoglobulins.

Cat. No.: HY-K0231 Anti-c-Myc Affinity Gel

MCE Anti-c-Myc Affinity Gel can be used for the detection and purification of c-Myc fusion expressed proteins, and immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

Cat. No.: HY-K0232 Anti-HA Affinity Gel

MCE Anti-HA Affinity Gel can be employed for the detection and purification of HA fusion expressed proteins, and can also be used in immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

Cat. No.: HY-K0233 Anti-RFP Affinity Gel

MCE Anti-RFP Affinity Gel can be used for the detection and purification of native RFP, RFP mutants, and IP assays.

Cat. No.: HY-K0235 Anti-YFP Affinity Gel

MCE Anti-YFP Affinity Gel can be used for the detection and purification of proteins tagged with GFP and EGFP and IP assays.

Cat. No.: HY-K0229 Anti-GFP Affinity Gel

MCE Anti-GFP Affinity Gel can be used for the detection and purification of GFP, EGFP, their fusion-expressed proteins and IP assays.

Cat. No.: HY-K0238 Ni-IDA Agarose

MCE Ni-IDA Agarose can be used for the detection and purification of His-tag proteins.

Cat. No.: HY-K0251 Protein A Agarose (Alkali-Tolerant) 4FF

MCE Protein A Agarose (Alkali-Tolerant) 4FF can efficiently binds IgG from serum, ascites, culture supernatants, and other antibody-containing samples, enabling high-performance antibody purification.

Cat. No.: HY-K0252 MBP Agarose (Dextrin) 6FF

MCE MBP Agarose (Dextrin) 6FF is prepared by covalently coupling dextrin to an agarose matrix. It features high binding capacity, excellent specificity, and superior ligand stability. It can achieve one-step purification of MBP fusion protein.

Cat. No.: HY-K0253 Heparin Agarose 6FF

MCE Heparin Agarose 6FF is suitable for the separation and purification of heparin-binding biomolecules, including antithrombin III, coagulation factors, other plasma proteins, DNA-binding proteins, lipoproteins, protein synthesis factors, nucleic acid-related enzymes, and steroid receptors.

Cat. No.: HY-K0254 Blue Agarose 6FF

MCE Blue Agarose 6FF can be used for the purification of biological macromolecules such as albumin, interferons, nucleotide-dependent enzymes (e.g., kinases, dehydrogenases), α2-macroglobulin, and coagulation factors.

Cat. No.: HY-K0255 IMAC Agarose (NTA) 6FF

MCE IMAC Agarose (NTA) 6FF is prepared by covalently coupling tetradentate nitrilotriacetic acid (NTA) to an agarose matrix. It features high binding capacity, excellent specificity, and superior ligand stability. The resin allows flexible chelation with metal ions such as Zn2+, Ni2+, or Cu2+, and is suitable for the purification of His-tagged recombinant proteins expressed in bacterial, mammalian, insect, and baculovirus systems.

Cat. No.: HY-K0256 Chelating Agarose (IDA) 6FF

MCE Chelating Agarose (IDA) 6FF allows flexible chelation with metal ions such as Zn2+, Ni2+, or Cu2+, and is suitable for the purification of His-tagged recombinant proteins expressed in bacterial, mammalian, insect, and baculovirus systems.

Cat. No.: HY-K0257 CM Agarose 6FF

MCE CM Agarose 6FF is a weak cation exchanger formed by covalently coupling carboxymethyl (CM) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

Cat. No.: HY-K0258 SP Agarose 6FF

MCE SP Agarose 6FF is a strong cation exchanger formed by covalently coupling sulfopropyl (SP) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

Cat. No.: HY-K0259 DEAE Agarose 6FF

MCE DEAE Agarose 6FF is a weak cation exchanger formed by covalently coupling diethylaminoethyl (DEAE) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

Cat. No.: HY-K0260 Q Agarose 6FF

MCE Q Agarose 6FF is a strong cation exchanger formed by covalently coupling quarternary ammonium (Q) groups to agarose. It is suitable for both laboratory research and large-scale industrial purification processes.

Cat. No.: HY-K0261 SP Agarose HP

MCE SP Agarose HP is a high-resolution strong cation exchanger formed by covalently coupling sulfopropyl (SP) groups to agarose. This medium offers high binding capacity, high specificity, and excellent ligand stability, making it suitable for both laboratory-scale and large-scale industrial purification applications.

Cat. No.: HY-K0262 DEAE Agarose HP

MCE DEAE Agarose HP is a high-resolution weak anion exchanger formed by covalently coupling diethylaminoethyl (DEAE) groups to agarose. This medium offers high binding capacity, high specificity, and excellent ligand stability, making it suitable for both laboratory-scale and large-scale industrial purification applications.

Cat. No.: HY-K0263 Q Agarose HP

MCE Q Agarose HP is a high-resolution strong anion exchanger formed by covalently coupling quaternary ammonium (Q) groups to agarose. This medium offers high binding capacity, high specificity, and excellent ligand stability, making it suitable for both laboratory-scale and large-scale industrial purification applications.

Cat. No.: HY-K0264 Butyl Agarose 4FF

MCE Butyl Agarose 4FF is a hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

Cat. No.: HY-K0265 Butyl Agarose HP

MCE Butyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

Cat. No.: HY-K0266 Octyl Agarose 6FF

MCE Octyl Agarose 6FF is a hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

Cat. No.: HY-K0267 Octyl Agarose HP

MCE Octyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling octyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

Cat. No.: HY-K0268 Phenyl Agarose (Low Sub) 6FF

MCE Phenyl Agarose (Low Sub) 6FF is a low-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

Cat. No.: HY-K0269 Phenyl Agarose (High Sub) 6FF

MCE Phenyl Agarose (High Sub) 6FF is a high-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

Cat. No.: HY-K0270 Phenyl Agarose HP

MCE Phenyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules.

Equilibration Buffer (2)

Cat. No.: HY-K1023 PBS Powder (1 L of 1×)

MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Cat. No.: HY-K1024 PBS Powder (2 L of 1×)

MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Magnetic Agarose Beads (8)

Cat. No.: HY-K0234 Glutathione Magnetic Agarose Beads

MCE Glutathione Magnetic Agarose Beads have high protein-binding capacity and stability, making it ideal for high performance purification of GST-tagged fusion proteins expressed in E. coli, yeast, insect and mammalian expression systems.

Cat. No.: HY-K0236 Anti-Flag Magnetic Agarose Beads

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of Flag (DYKDDDDK) fusion-expressed proteins and IP assays.

Cat. No.: HY-K0237 Anti-HA Magnetic Agarose Beads

MCE Anti-HA Magnetic Agarose Beads can be used for the detection and purification of HA fusion-expressed proteins and IP assays.

Cat. No.: HY-K0240 Ni-IDA Magnetic Agarose Beads

MCE Ni-IDA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

Cat. No.: HY-K0241 Ni-NTA Magnetic Agarose Beads

MCE Ni-NTA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

Cat. No.: HY-K0242 Protein A Plus Magnetic Agarose Beads

MCE Protein A Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

Cat. No.: HY-K0243 Protein G Plus Magnetic Agarose Beads

MCE Protein G Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

Cat. No.: HY-K0246 Anti-GFP Magnetic Agarose Beads

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of GFP, EGFP, and their fusion-expressed proteins and IP assays.