OL-FS13 alleviates experimental cerebral ischemia-reperfusion injury

  • Exp Neurol. 2022 Nov;357:114180. doi: 10.1016/j.expneurol.2022.114180.
Naixin Liu  1 ,  Yilin Li  1 ,  Ying Yang  2 ,  Longjun Shu  3 ,  Yixiang Liu  3 ,  Yutong Wu  1 ,  Dandan Sun  1 ,  Zijian Kang  1 ,  Yue Zhang  1 ,  Dan Ni  1 ,  Ziqi Wei  1 ,  Shanshan Li  1 ,  Meifeng Yang  1 ,  Ying Wang  4 ,  Jun Sun  5 ,  Xinwang Yang  6
Affiliations
  • 1. Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China.
  • 2. Endocrinology Department of affiliated Hospital of Yunnan University, Kunming 650021, Yunnan, China.
  • 3. Key Laboratory of Chemistry in Ethnic Medicinal Resources & Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes, State Ethnic Affairs Commission & Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650504, Yunnan, China.
  • 4. Key Laboratory of Chemistry in Ethnic Medicinal Resources & Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes, State Ethnic Affairs Commission & Ministry of Education, School of Ethnic Medicine, Yunnan Minzu University, Kunming 650504, Yunnan, China. Electronic address: [email protected].
  • 5. Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: [email protected].
  • 6. Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming 650500, Yunnan, China. Electronic address: [email protected].
PMID: 35901974 DOI: 10.1016/j.expneurol.2022.114180
Abstract

Cerebral ischemia-reperfusion (I/R) is the main cause of neurological injury after stroke. However, existing treatments for I/R injury are relatively poor, and relevant drugs need to be further explored. Amphibians have received increasing attention as a resource bank of bioactive peptides. However, reports on neuroprotective peptides from amphibians remain extremely rare. Here, we identified a new neuroprotective peptide (OL-FS13, amino acid sequence: FSLLLTWWRRRVC) from the odorous frog species Odorrana livida using a constructed cDNA library. OL-FS13 significantly improving infarct volume, behavioral and histological abnormalities in rats, and also showed neuroprotective activities in PC12 cell (by oxygen glucose deprivation/reoxygenation, OGD/R). Mechanistically, OL-FS13 increased the level of antioxidative Enzymes to resist oxidative stress and alleviated endoplasmic reticulum (ER) stress induced by I/R and OGD/R. The use of ML385 (Nrf2 inhibitor) indicated that OL-FS13 relieved nerve damage caused by oxidative and ER stress by increasing the nuclear displacement of Nrf2. Collectively, this research provides a novel drug candidate for the clinical cerebral I/R curation.

Keywords
Bioactive peptide; Cerebral ischemia; Neuroprotection; Oxidative stress; Reperfusion.
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