A simple electrical stimulation cell culture system on the myelination of dorsal root ganglia and Schwann cells

  • Biotechniques. 2019 Jul;67(1):11-15. doi: 10.2144/btn-2018-0175.
Zhuowen Liang  1 ,  Tao Lei  2 ,  Shuang Wang  3 ,  ZhuoJing Luo  1 ,  XueYu Hu  1
Affiliations
  • 1. Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
  • 2. School of Biomedical Engineering, The Fourth Military Medical University, Xi'an, China.
  • 3. Institute of Photonics & Photon-Technology, Northwest University, Xi'an, China.
PMID: 31124698 DOI: 10.2144/btn-2018-0175
Abstract

A large number of animal experiments and clinical trials have confirmed that electrical stimulation can accelerate the growth of axons and recovery of motor function, all of which are inseparable from the formation of myelin. Therefore, establishment of a suitable electrical stimulation platform to study the effects of electrical stimulation on the myelin process of dorsal root ganglia and Schwann cells is of great significance for understanding the recovery of electrical stimulation. We designed a simple conductive glass Cell Culture system to overcome the shortcomings of direct contact of the electrode with the culture solution, and the number of culture chambers can be selected based on the purpose of the experiment in order to reduce experimental time and cost.

Keywords
Schwann cells; co-culture; conductive glass; dorsal root ganglion; electrical stimulation; myelination.
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