The novel gene fad104, containing a fibronectin type III domain, has a significant role in adipogenesis

  • FEBS Lett. 2004 Nov 5;577(1-2):49-54. doi: 10.1016/j.febslet.2004.09.062.
Kei Tominaga  1 ,  Chiharu Kondo ,  Yoshikazu Johmura ,  Makoto Nishizuka ,  Masayoshi Imagawa
Affiliations
  • 1. Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
PMID: 15527760 DOI: 10.1016/j.febslet.2004.09.062
Abstract

A novel gene named fad104 (factor for adipocyte differentiation-104), whose expression level quickly increased in the early stage of adipogenesis, was isolated and characterized. The deduced amino acid sequence of fad104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. The expression of fad104 was detected in adipocyte differentiable 3T3-L1 cells but not observed in the non-adipogenic cell line NIH-3T3. Moreover, the ability of 3T3-L1 cells to differentiate declined with the knockdown of fad104 by RNA interference, strongly indicating that fad104 functions as a positive regulator of adipogenesis.