2. Academic Validation
  3. Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)

Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)

  • Front Neuroanat. 2018 Mar 27:12:23. doi: 10.3389/fnana.2018.00023.
Karen Stanic 1,  Alonso Quiroz 1,  Carmen G Lemus 2,  Ignacio A Wichmann 3 4,  Alejandro H Corvalán 3 4 5,  Gareth I Owen 1 3 4 5 6,  Juan C Opazo 7,  Miguel L Concha 2 8,  Julio D Amigo 1
Affiliations

Affiliations

  • 1 Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • 2 Anatomy and Developmental Biology Program, Faculty of Medicine, Institute of Biomedical Sciences, Universidad de Chile, Santiago, Chile.
  • 3 Advanced Center for Chronic Diseases, Santiago, Chile.
  • 4 UC Center for Investigation in Oncology, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • 5 Departamento de Oncología y Hematología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
  • 6 Millennium Institute on Immunology and Immunotherapy, Santiago, Chile.
  • 7 Instituto de Ciencias Ambientales y Evolutivas, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
  • 8 Biomedical Neuroscience Institute, Universidad de Chile, Santiago, Chile.
PMID: 29636669 DOI: 10.3389/fnana.2018.00023
Abstract

The Reprimo (RPRM) family is composed of highly conserved single-exon genes. The expression pattern of this gene family has been recently described during zebrafish (Danio rerio) embryogenesis, and primarily locates in the nervous system. Its most characterized member, RPRM, which duplicated to give rise rprma and rprmb in the fish lineage, is known to act as a tumor-suppressor gene in mammalian models. Here, we describe in detail the spatiotemporal expression of three rprm genes (rprma, rprmb, and rprml) within distinct anatomical structures in the developing peripheral and central nervous system. In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. Most importantly, the expression of RPRM has been conserved between teleosts and mammals. Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family.

Keywords

neurogenesis; olfactory systems; opticum tectum; reprimo; zebrafish (Danio rerio).

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