A simple and rapid LC-MS/MS method for quantitation of luseogliflozin in rat plasma and its application to a PK study

  • Bioanalysis. 2017 Jan;9(2):163-171. doi: 10.4155/bio-2016-0188.
Shinji Kobuchi  1 ,  Megumi Matsuno  1 ,  Momoko Kawamoto  1 ,  Naoto Kojima  2 ,  Yukako Ito  1 ,  Masayuki Yamashita  2 ,  Toshiyuki Sakaeda  1
Affiliations
  • 1. Department of Pharmacokinetics, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
  • 2. Department of Pharmaceutical Manufacturing Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
PMID: 27960548 DOI: 10.4155/bio-2016-0188
Abstract

Aim: Luseogliflozin is a novel sodium-dependent glucose cotransporter-2 inhibitor for the treatment of Type 2 diabetes mellitus. To assist pharmacokinetic and toxicodynamic studies, a rapid LC-MS/MS method were developed and validated for the quantitation of luseogliflozin in rat plasma.

Results: Sample preparation was carried out using simplified protein precipitation and liquid-liquid extraction procedures, and the run time was only 4 min. Extraction recovery was 92.9 to 95.3%, and the method was validated over the range 0.5 to 500 ng/ml for luseogliflozin with acceptable specificity, accuracy and precision.

Conclusion: The validated method is considered suitable to quantify luseogliflozin in pharmacokinetic and pharmacodynamic/toxicodynamic studies in rats.

Keywords
LC–MS/MS; SGLT2 inhibitors; antidiabetic agent; dapagliflozin; luseogliflozin; pharmacokinetics; rat plasma.
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