MLF1 (Myeloid leukemia factor 1) Antibody (YA8197)(PBS only)

Cat. No.: HY-P88513A: Data Sheet User Guide for Antibodies
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MLF1 (Myeloid leukemia factor 1) Antibody (YA8197) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MLF1 (Myeloid leukemia factor 1).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MLF1 (Myeloid leukemia factor 1) Antibody (YA8197) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MLF1 (Myeloid leukemia factor 1).

Host

Mouse

Molecular Weight

Predicted band size: 30.4 kDa

Species Reactivity

Human

SwissProt ID

P58340

Immunogen

Full length human recombinant protein of human MLF1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus

Subcellular Localization：Cytoplasm,Nucleus,Cell projection, cilium,Cytoplasm, cytoskeleton, cilium basal body

Expression：
Tissue_Specificity: Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes

Isoforms & Post-Translational Modification：P58340 has 5 isomers: P58340-1: 30627 Da (predicted); P58340-2: 23117 Da (predicted); P58340-3: 33888 Da (predicted); P58340-5: 27625 Da (predicted); P58340-4: 29095 Da (predicted).
Phosphorylation is required for binding to YWHAZ

Subunit：Interacts with CENPU

Synonyms

myeloid leukemia factor 1; myeloid leukemia factor 1 variant 1; myeloid leukemia factor 1 variant 2; myeloid leukemia factor 1 variant 3

Documentation

User Guide for Antibodies (1077 KB)