β-Sitosterol Suppresses Lipopolysaccharide-Induced Inflammation and Lipogenesis Disorder in Bovine Mammary Epithelial Cells

  • Int J Mol Sci. 2023 Sep 27;24(19):14644. doi: 10.3390/ijms241914644.
Yating Fan  1 ,  Jinglin Shen  1 ,  Xinlu Liu  1 ,  Junhao Cui  1 ,  Jiayi Liu  1 ,  Dongqiao Peng  1 ,  Yongcheng Jin  1
Affiliations
  • 1. Jilin Provincial Key Laboratory of Livestock and Poultry Feed and Feeding in Northeastern Frigid Area, Department of Animal Science, College of Animal Science, Jilin University, Changchun 130062, China.
PMID: 37834091 DOI: 10.3390/ijms241914644
Abstract

β-sitosterol, a natural plant steroid, has been shown to promote anti-inflammatory and antioxidant activities in the body. In this study, β-sitosterol was used to protect against lipopolysaccharide (LPS)-induced cell damage in bovine mammary epithelial cells, which are commonly studied as a cell model of mammary inflammatory response and lipogenesis. Results showed that treatment with a combination of LPS and β-sitosterol significantly reduced oxidative stress and inflammation, while increasing the expression of anti-apoptotic proteins and activating the hypoxia-inducible factor-1(HIF-1α)/mammalian target of rapamycin(mTOR) signaling pathway to inhibit Apoptosis and improve lipid synthesis-related gene expression. Our finding suggests that β-sitosterol has the potential to alleviate inflammation in the mammary gland.

Keywords
HIF-1α signaling pathway; LPS; mTOR signaling pathway; mastitis; β-sitosterol.
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