Free and Bound Phenolics of Laird Lentil ( Lens culinaris) Hulls and the Anti-inflammatory Activity of their Digestive Products via Crosstalk between NF-κB and Keap1-Nrf2 Signaling Pathways in HT-29 Cells

  • J Agric Food Chem. 2022 Oct 5. doi: 10.1021/acs.jafc.2c04471.
Fanghua Guo  1 ,  Li Peng  1 ,  Hua Xiong  1 ,  Jiaqi Wang  2 ,  Rong Tsao  3 ,  Xin Peng  4 ,  Li Jiang  5 ,  Yong Sun  1
Affiliations
  • 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
  • 2. Ausnutria Institute of Food and Nutrition, Ausnutria Dairy (China) Co. Ltd., Changsha 410219, China.
  • 3. Guelph Research and Development Centre, Agricultural and Agri-Food Canada, 93 Stone Road West, Guelph, ON N1G 5C9, Canada.
  • 4. Ningbo Municipal Hospital of TCM, Affiliated Hospital of Zhejiang Chinese Medical University, Ningbo 315010, China.
  • 5. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi, China.
PMID: 36196880 DOI: 10.1021/acs.jafc.2c04471
Abstract

Polyphenol-rich Laird lentil hulls are a byproduct of lentil processing. In the present study, free and bound Polyphenols in lentil hulls were analyzed with UHPLC-LTQ-OrbiTrap-MS2, and the anti-inflammatory mechanism of their digestive products was explored based on the NF-κB and Keap1-Nrf2 signaling pathways in the HT-29 cell model. In summary, a total of 27 Polyphenols and 5 nonphenolic constituents were identified in free and bound fractions, and among them, catechin glucoside, kaempferol tetraglucoside, procyanidin dimer, and dihydroxybenzoic acid-O-dipentoside were the main Polyphenols in the digestive products. These digestive products could reduce inflammatory mediators and exert anti-inflammatory activity by inhibiting NF-κB and activating Keap1-Nrf2 signaling pathways, and there was crosstalk between them, which was a mutual inhibition effect. The results show that Polyphenols in lentil hulls are a good source of anti-inflammatory ingredients and have a promising development potential.

Keywords
Keap1-Nrf2; Laird lentil hulls; NF-κB; crosstalk; digestive products; free and bound polyphenols.
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