C-MPL Protein, Cynomolgus (HEK293, His)

Customer Review

Based on 1 Customer Validation

C-MPL protein is a key member of the type I cytokine receptor family and is essential for mediating cellular responses to various cytokines. Its research enhances our understanding of cell signaling, providing potential applications for therapeutic intervention in hematopoietic disorders and immune disorders. C-MPL Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived C-MPL protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Cynomolgus
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

C-MPL protein is a key member of the type I cytokine receptor family and is essential for mediating cellular responses to various cytokines. Its research enhances our understanding of cell signaling, providing potential applications for therapeutic intervention in hematopoietic disorders and immune disorders. C-MPL Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived C-MPL protein, expressed by HEK293 , with C-His labeled tag.

Background

The C-MPL Protein is a pivotal member of the type I cytokine receptor family, specifically falling under the Type 1 subfamily, underlining its crucial role in mediating cellular responses to various cytokines. As part of this receptor family, C-MPL likely shares conserved structural and functional features with related receptors, emphasizing its involvement in transducing signals from specific type I cytokines. The classification within the type I cytokine receptor family underscores its specific designation within the broader context of cell signaling, providing insights into its unique contributions to hematopoiesis and immune regulation. The study of C-MPL contributes to our understanding of its role in physiological processes, offering potential applications in therapeutic interventions for conditions related to hematopoietic disorders and immune dysregulation. Further exploration of C-MPL's role holds promise for enhancing our knowledge of its contributions to both normal cellular function and pathological conditions.

Verified Bioactivity

Immobilized Cynomolgus c-MPL, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-C-MPL Antibody, hFc Tag with the EC50 of ≤12.7 ng/mL determined by ELISA.

Technical Parameters

  • Species Cynomolgus
  • Source HEK293
  • Tag C-8*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • C-MPL (Q22-T489)
      Accession # A0A2K5UGC7
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    TPO-R; CD110; MPL; c-Mpl; MPLV; K12

  • AA Sequence

    QVSSQDVSLLASDSEPLKCFSRTFEDLTCFWDEEEAAPSGTYQLLYAYPGEKPRACPLSSQSVPRFGTRYVCQFPAQEEVRLFSPLHLWVKNVFLNQTQIQRVLFVDSVGLPAPPSIIKAMGGSQPGELQISWEAPAPEISDFLRYELRYGPKDLKNSTGPTVIQLIATETCCPALQRPHSASALDQSPCAQPTMPWQDGPKQTSPTREASALTAVGGSCLISGLQPGNSYWLQLRSEPDGISLGGSWGSWSLPVTVDLPGDAVAIGLQCFTLDLKNVTCQWQQEDHARSQGFFYHSRARCCPRDRYPIWEDCEEEEKTNPGLQTPQFSRCHFKSRNDSVIHILVEVTTALGAVHSYLGSPFWIHQAVRLPTPNLHWREISSGHLELEWQHPSSWAAQETCYQLRYTGEGHQDWKVLEPPLGARGGTLELRPRSRYRLQLRARLNGPTYQGPWSSWSDPARVETATET

  • Predicted Molecular Mass

    53.5 kDa

  • Molecular Weight

    Approximately 60-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)
=
Mass (in vial)
÷
Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start)
×
Volume (start)
=
Concentration (final)
×
Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL