Cy5-UTP
Cy5-UTP is a fluorescently labeled ribonucleotide triphosphate that can be used as a substrate for terminal deoxynucleotidyl transferase (TdT). Cy5-UTP can be employed to label RNA probes generated in vitro (Ex/Em: 650/665 nm). Cy5-UTP can be applied in FISH, multi-color fluorescence analysis, especially in dual-color expression arrays that combine with Cy5-UTP.
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- Fòrmula: C45H58N5O22P3S2
- Peso molecular:1178.01
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Cy5-UTP (100 μM; 1 h) shows minimal bias toward the base composition of immobilized hexamer oligonucleotide probes, making it well suited for quantitative analysis of microarray spots where probes are coupled via a 5' end linkage to the solid phase[1].
Cy5-UTP (0.1-10% of total UTP pool) enables controllable, high-yield fluorescent co-transcriptional body labeling of FLuc mRNA via T7 RNA polymerase, with a linear relationship between Cy5-UTP input fraction and fluorophores per RNA, and 0.79% Cy5-UTP yielding 5 labels per mRNA[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Peso molecular 1178.01
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Fòrmula C45H58N5O22P3S2
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SMILES
OP(OP(OP(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=C(/C=C/CNC(CCCCCN(/C(C3(C)C)=C/C=C/C=C/C4=[N+](CC)C5=C(C4(C)C)C=C(S([O-])(=O)=O)C=C5)C6=C3C=C(S(O)(=O)=O)C=C6)=O)C(NC2=O)=O)O1)(O)=O)(O)=O)(O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Guerra CE, et al. Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase. Biotechniques. 2006;41(1):53-56. [Content Brief]
[2]. Custer TC, et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. Protein Sci. 2017;26(7):1363-1379. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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