Erythrosine B
Based on 1 Customer Validation
Erythrosine B is an artificial dye widely used in the food and textile industries. Erythrosine B is also a novel photosensitizer which has been used to develop animal models.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 16423-68-0
- Formula: C20H6I4Na2O5
- Molecular Weight:879.86
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Only the two highest concentrations of Erythrosine B tested (50.0 and 70.0 μg/mL) are significantly different (p<0.05) from the vehicle control group when the Tail Moment and Tail Intensity, which represent the extent of DNA damage, are analyzed. Results show increased micronuclei (MNi) frequencies at six of the seven Erythrosine B concentrations (0.2 to 70.0 μg/mL) when compared to vehicle control group[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 16423-68-0
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Appearance Solid
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Molecular Weight 879.86
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Formula C20H6I4Na2O5
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Color Pink to red
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SMILES
O=C1OC2(C3=C(OC4=C2C=C(I)C(O[Na])=C4I)C(I)=C(O[Na])C(I)=C3)C5=C1C=CC=C5
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Synonyms
Erythrosin extra bluish
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 33.33 mg/mL (37.88 mM; Need ultrasonic)
DMSO : 33.33 mg/mL (37.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
The alkaline single-cell gel electrophoresis assay (comet assay) is performed. Briefly, 2×105 HepG2 cells are seeded in a 24-well plate for 24 h. The cells are then treated with Erythrosine B at 0.1, 0.2, 2.0, 10.0, 25.0, 50.0 or 70.0 μg/mL (final concentration) for 4 h; vehicle control (0.7% DMSO) and positive control (doxorubicin 0.3 μg/mL) treatments are also performed. The HepG2 cell suspension is mixed with 37°C low-melting point agarose and transferred to normal-melting point agarose-coated slides. One hundred randomly chosen nucleoids are analyzed per treatment, and a total of three independent experiments are performed[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen W, et al. Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B. Int J Ophthalmol. 2014 Apr 18;7(2):232-8. [Content Brief]
[2]. Chequer FM, et al. Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem Toxicol. 2012 Oct;50(10):3447-51. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.1365 mL | 5.6827 mL | 11.3654 mL | 28.4136 mL |
| 5 mM | 0.2273 mL | 1.1365 mL | 2.2731 mL | 5.6827 mL | |
| 10 mM | 0.1137 mL | 0.5683 mL | 1.1365 mL | 2.8414 mL | |
| 15 mM | 0.0758 mL | 0.3788 mL | 0.7577 mL | 1.8942 mL | |
| 20 mM | 0.0568 mL | 0.2841 mL | 0.5683 mL | 1.4207 mL | |
| 25 mM | 0.0455 mL | 0.2273 mL | 0.4546 mL | 1.1365 mL | |
| 30 mM | 0.0379 mL | 0.1894 mL | 0.3788 mL | 0.9471 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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