Oleosin Protein, Glycine max (Cell-Free, His)

Oleosins are essential for stabilizing liposomes during seed drying, preventing oil coalescence and underscoring their importance in seed physiology. It binds to lipid and phospholipid moieties, suggesting a multifunctional molecular interaction that contributes to liposome integrity. Oleosin Protein, Glycine max (Cell-Free, His) is the recombinant Oleosin protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

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  • Species: Others
  • Source: E. coli Cell-free
  • 保管条件:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • Help & FAQs

生物活性

製品説明

Oleosins are essential for stabilizing liposomes during seed drying, preventing oil coalescence and underscoring their importance in seed physiology. It binds to lipid and phospholipid moieties, suggesting a multifunctional molecular interaction that contributes to liposome integrity. Oleosin Protein, Glycine max (Cell-Free, His) is the recombinant Oleosin protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

Background

The Oleosin protein plays a crucial structural role in stabilizing lipid bodies during seed desiccation by preventing the coalescence of oil, underscoring its significance in seed physiology. It likely engages with both lipid and phospholipid moieties of lipid bodies, suggesting a versatile molecular interaction that contributes to the integrity and function of the lipid body. Additionally, Oleosin may serve as a provider of recognition signals for specific lipase anchorage, indicating its involvement in the regulation of lipolysis during seedling growth. The multifaceted functions associated with Oleosin highlight its pivotal role in governing essential processes related to lipid metabolism and seed development.

Technical Parameters

  • Species Others
  • Source E. coli Cell-free
  • Tag N-10*His
  • アクセッション番号
  • 遺伝子ID
  • Molecular Construction
    • N-term
    • 10*His
    • Oleosin (M1-S147)
      Accession # C6SZ13
    • C-term
  • Protein Length

    Full Length

  • 別名

    Oleosin

  • AA Sequence

    MAELHYQPQHQYPLRYPNDPHQQTRSSTHQVVKAATAVTAGGSLLILASLILAATVIALTIVTPLFVIFSPVLVPAVITVALLSLGFLASSGFGVAAITVLAWIYRYVTGKQPPGADQLDSARHKIMDKAREIKDYGQQQISGVQAS

  • Predicted Molecular Mass

    17.3 kDa

  • 純度

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

性状

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

輸送条件

Room temperature in continental US; may vary elsewhere.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)
=
Mass (in vial)
÷
Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始)
×
体積 (開始)
=
濃度 (終了)
×
体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL