2. Academic Validation
  3. miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b

miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b

  • FEBS Lett. 2009 Jul 7;583(13):2263-8. doi: 10.1016/j.febslet.2009.06.006.
Yosuke Mizuno 1,  Yoshimi Tokuzawa,  Yuichi Ninomiya,  Ken Yagi,  Yukiko Yatsuka-Kanesaki,  Tatsuo Suda,  Toru Fukuda,  Takenobu Katagiri,  Yasumitsu Kondoh,  Tomoyuki Amemiya,  Hideo Tashiro,  Yasushi Okazaki
Affiliations

Affiliation

  • 1 Division of Functional Genomics and Systems Medicine, Research Center for Genomic Medicine, Saitama Medical University, Hidaka, Japan.
PMID: 19520079 DOI: 10.1016/j.febslet.2009.06.006
Abstract

Although MicroRNAs (miRNAs) are involved in many biological processes, the mechanisms whereby miRNAs regulate osteoblastic differentiation are poorly understood. Here, we found that BMP-4-induced osteoblastic differentiation of bone marrow-derived ST2 stromal cells was promoted and repressed after transfection of sense and antisense miR-210, respectively. A reporter assay demonstrated that the Activin A receptor type 1B (AcvR1b) gene was a target for miR-210. Furthermore, inhibition of transforming growth factor-beta (TGF-beta)/activin signaling in ST2 cells with SB431542 promoted osteoblastic differentiation. We conclude that miR-210 acts as a positive regulator of osteoblastic differentiation by inhibiting the TGF-beta/activin signaling pathway through inhibition of AcvR1b.

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