2. NF-κB Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation
  3. Keap1-Nrf2 Apoptosis Ferroptosis Reactive Oxygen Species (ROS)
  4. Nrf2 activator-25

Nrf2 activator-25 is a Nrf2 activator. Nrf2 activator-25 promotes the dissociation of Nrf2 from Keap1 (with a Kd of 21.3 μM for Keap1), drives Nrf2 nuclear translocation, and increases the production of downstream antioxidant enzymes. Nrf2 activator-25 inhibits apoptosis, ferroptosis and vascular fibrosis, and protects vascular endothelial cells from damage. Nrf2 activator-25 can be used in the research of diabetic vascular diseases.

For research use only. We do not sell to patients.

Nrf2 activator-25

Nrf2 activator-25 Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Nrf2 activator-25 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Nrf2 activator-25 is a Nrf2 activator. Nrf2 activator-25 promotes the dissociation of Nrf2 from Keap1 (with a Kd of 21.3 μM for Keap1), drives Nrf2 nuclear translocation, and increases the production of downstream antioxidant enzymes. Nrf2 activator-25 inhibits apoptosis, ferroptosis and vascular fibrosis, and protects vascular endothelial cells from damage. Nrf2 activator-25 can be used in the research of diabetic vascular diseases[1].

In Vitro

Nrf2 activator-25 (Compound 3h) at 20 μM maintains the survival rate of serum- and growth factor-starved human umbilical vein endothelial cells (HUVECs) at 93.61%, with extremely low cytotoxicity[1].
Nrf2 activator-25 (5-20 μM; 6-24 h) concentration-dependently inhibits apoptosis and ferroptosis of HUVECs damaged by ox-LDL, high glucose and CoCl2 after 12 h of treatment[1].
Nrf2 activator-25 (5-20 μM; 6-12 h) concentration-dependently inhibits the expression of ICAM-1 and VCAM-1 in HUVECs damaged by ox-LDL, high glucose and CoCl2[1].
Nrf2 activator-25 (20 μM; 6-24 h) protects HUVECs injured by ox-LDL, high glucose and CoCl2 against mitochondrial membrane potential loss[1].
Nrf2 activator-25 (20 μM; 6-24 h) inhibits ROS accumulation in HUVECs damaged by ox-LDL, high glucose and CoCl2[1].
Nrf2 activator-25 (5-20 μM; 6-24 h) concentration-dependently inhibits lipid peroxidation in HUVECs damaged by ox-LDL, high glucose, and CoCl2, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nrf2 activator-25 Related Antibodies

Apoptosis Analysis[1]

Cell Line: ox-LDL-injured human umbilical vein endothelial cells (HUVECs)
Concentration: 5, 10 and 20 μM
Incubation Time: 6 h
Result: Reduced the percentage of apoptotic HUVECs with condensed/fragmented nuclei in a concentration-dependent manner, relative to ox-LDL-only treated cells.

Apoptosis Analysis[1]

Cell Line: high glucose (HG)-injured human umbilical vein endothelial cells (HUVECs)
Concentration: 5, 10 and 20 μM
Incubation Time: 24 h
Result: Reduced the percentage of apoptotic HUVECs with condensed/fragmented nuclei in a concentration-dependent manner, relative to HG-only treated cells.

Apoptosis Analysis[1]

Cell Line: CoCl2-injured human umbilical vein endothelial cells (HUVECs)
Concentration: 5, 10 and 20 μM
Incubation Time: 12 h
Result: Reduced the percentage of apoptotic HUVECs with condensed/fragmented nuclei in a concentration-dependent manner, relative to CoCl2-only treated cells.

Western Blot Analysis[1]

Cell Line: ox-LDL-injured human umbilical vein endothelial cells (HUVECs)
Concentration: 5, 10 and 20 μM
Incubation Time: 6 h
Result: Concentration-dependently reduced the ox-LDL-induced upregulation of ICAM-1 and VCAM-1 protein levels, with greater efficacy than tBHQ.

Western Blot Analysis[1]

Cell Line: high glucose (HG)-injured human umbilical vein endothelial cells (HUVECs)
Concentration: 5, 10 and 20 μM
Incubation Time: 24 h
Result: Concentration-dependently reduced the HG-induced upregulation of ICAM-1 and VCAM-1 protein levels, with greater efficacy than tBHQ.

Western Blot Analysis[1]

Cell Line: CoCl2-injured human umbilical vein endothelial cells (HUVECs)
Concentration: 5, 10 and 20 μM
Incubation Time: 12 h
Result: Concentration-dependently reduced the CoCl2-induced upregulation of ICAM-1 and VCAM-1 protein levels, with greater efficacy than tBHQ.
In Vivo

Nrf2 activator-25 (Compound 3h) (1 mg/kg; i.p.; daily; 8 weeks) robustly attenuates endothelial damage and vascular fibrosis in STZ (HY-13753)-induced diabetic mice, while reducing apoptosis, lipid peroxidation, and restoring glutathione levels[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (male, 6-8 weeks old, STZ-induced diabetes)[1]
Dosage: 1 mg/kg
Administration: i.p.; daily; 8 weeks
Result: Significantly alleviated intimal damage and vascular fibrosis, with lower relative fibrosis severity compared to the STZ-only group.
Reduced the proportion of apoptotic cells in the thoracic aortic endothelium compared to the STZ-only group.
Decreased aortic tissue MDA and LPO content relative to the STZ-only group.
Increased aortic tissue GSH content relative to the STZ-only group.
Molecular Weight

259.30

Formula

C15H17NO3
SMILES

O=C(N1C=CC=C1)OC2=CC=C(O)C(C(C)(C)C)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Nrf2 activator-25 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nrf2 activator-25Keap1-Nrf2ApoptosisFerroptosisReactive Oxygen Species (ROS)Keap1vascular endothelial cellsVCAM-1ferroptosisdiabetic vascular diseaseNrf2apoptosisHUVECsvascular fibrosisICAM-1Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Nrf2 activator-25
Cat. No.:
HY-183353
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.