Diazinon toxicity in hepatic and spleen mononuclear cells is associated to early induction of oxidative stress

  • Int J Environ Health Res. 2022 Oct;32(10):2309-2323. doi: 10.1080/09603123.2021.1962814.
Manuel Iván Girón-Pérez  1 ,  Verónica S Mary  2  3 ,  Héctor R Rubinstein  2  3 ,  Gladys A Toledo-Ibarra  1 ,  Martín G Theumer  2  3
Affiliations
  • 1. Universidad Autónoma de Nayarit,Secretaría de Investigación Y Posgrado, Laboratorio de Inmunotoxicología, Boulevard Tepic-Xalisco S/n, Cd de La Cultura Amado Nervo, C.P, Tepic, Nayarit, Mexico.
  • 2. Departamento De Bioquímica Clínica, Facultad De Ciencias Químicas, Universidad Nacional De Córdoba (UNC), Córdoba, Argentina.
  • 3. Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Centro de Investigaciones En Bioquímica Clínica E Inmunología (CIBICI), Córdoba, Argentina.
PMID: 34404283 DOI: 10.1080/09603123.2021.1962814
Abstract

Diazinon is an organophosphorus pesticide, which may have potential toxic effects on the liver and immune system; however, the underlying mechanisms remain mostly unidentified. This work is aimed at evaluating the oxidative stress and cell cycle alterations elicited by low-dose diazinon in a rat liver cell line (BRL-3A) and spleen mononuclear cells (SMC) from Wistar rats. Diazinon (10-50 μM) caused early Reactive Oxygen Species (ROS) generation (from 4 h) as well as increased O2•- level (from 0.5 h), which led to subsequent lipid peroxidation at 24 h, in BRL-3A cells. In SMC, diazinon (20 μM) produced similar increases in ROS levels, at 4 and 24 h, with the highest O2•- level being found at 4 h. Low-dose diazinon induced G1-phase arrest and cell death in hepatic cells and SMC. Therefore, diazinon could affect the liver and the immunological system through the premature oxidative stress induction.Abbreviations: O2•-: superoxide anion radical; ROS: reactive oxygen species; SMC: spleen mononuclear cells; TBARS: thiobarbituric acid reactive substances.

Keywords
Diazinon; Hepatotoxicity; ROS; immunotoxicity.
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