Adenosine Deaminase/ADA Protein, Human (sf9, His)

The adenosine deaminase/ADA protein catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine, which is critical for purine metabolism and adenosine homeostasis. It affects cell signaling indirectly by regulating extracellular adenosine. Adenosine Deaminase/ADA Protein, Human (sf9, His) is the recombinant human-derived Adenosine Deaminase/ADA protein, expressed by Sf9 insect cells , with C-His labeled tag.

Adenosine Deaminase (ADA) protein serves as a crucial enzyme, orchestrating the hydrolytic deamination of adenosine and 2-deoxyadenosine. This enzymatic activity is fundamental to purine metabolism and contributes significantly to adenosine homeostasis. Beyond its direct role in nucleotide catabolism, ADA plays a multifaceted role in cellular signaling events. It acts as a positive regulator of T-cell coactivation, influencing lymphocyte-epithelial cell adhesion through its interaction with DPP4. Additionally, ADA enhances dendritic cell immunogenicity, affecting costimulatory molecule expression and cytokine secretion. The protein also modulates adenosine receptors ADORA1 and ADORA2A, contributing to ligand affinity via conformational changes. Furthermore, ADA participates in plasminogen activation, influences CD4+ T-cell differentiation and proliferation, and plays roles in male fertility and early postimplantation embryonic development.

Measured by the ability to catalyze the hydrolytic deamination of adenosine to inosine. The specific activity is 129647 pmol/min/μg.

Human

Sf9 insect cells

C-His

P00813 (M1-L363)

100  [NCBI]

Full Length

MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL

Approximately 44 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.