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Adenosine Deaminase/ADA Protein, Human (sf9, His)

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The adenosine deaminase/ADA protein catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine, which is critical for purine metabolism and adenosine homeostasis. It affects cell signaling indirectly by regulating extracellular adenosine. Adenosine Deaminase/ADA Protein, Human (sf9, His) is the recombinant human-derived Adenosine Deaminase/ADA protein, expressed by Sf9 insect cells , with C-His labeled tag.

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  • Species: Human
  • Source: Sf9 insect cells
  • 보관:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Adenosine Deaminase/ADA Protein, Human (sf9, His) Product Image
  • Adenosine Deaminase/ADA Protein, Human (sf9, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
주변의 대리점 찾기 Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • 각종 서류
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Biological Activity

제품 설명

The adenosine deaminase/ADA protein catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine, which is critical for purine metabolism and adenosine homeostasis. It affects cell signaling indirectly by regulating extracellular adenosine. Adenosine Deaminase/ADA Protein, Human (sf9, His) is the recombinant human-derived Adenosine Deaminase/ADA protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

Adenosine Deaminase (ADA) protein serves as a crucial enzyme, orchestrating the hydrolytic deamination of adenosine and 2-deoxyadenosine. This enzymatic activity is fundamental to purine metabolism and contributes significantly to adenosine homeostasis. Beyond its direct role in nucleotide catabolism, ADA plays a multifaceted role in cellular signaling events. It acts as a positive regulator of T-cell coactivation, influencing lymphocyte-epithelial cell adhesion through its interaction with DPP4. Additionally, ADA enhances dendritic cell immunogenicity, affecting costimulatory molecule expression and cytokine secretion. The protein also modulates adenosine receptors ADORA1 and ADORA2A, contributing to ligand affinity via conformational changes. Furthermore, ADA participates in plasminogen activation, influences CD4+ T-cell differentiation and proliferation, and plays roles in male fertility and early postimplantation embryonic development.

Verified Bioactivity

Measured by the ability to catalyze the hydrolytic deamination of adenosine to inosine. The specific activity is 129647 pmol/min/μg.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    P00813 (M1-L363)

  • Gene ID
    100  [NCBI]
  • Molecular Construction
    • N-term
    • ADA (M1-L363)
      Accession # P00813
    • His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    Adenosine deaminase; ADA; ADA1

  • AA Sequence

    MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL

  • Predicted Molecular Mass

    42.1 kDa

  • 분자량

    Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

선적

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL

Adenosine Deaminase/ADA Protein, Human (sf9, His) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Adenosine Deaminase/ADA Protein, Human (sf9, His) Product Image