GPNMB/Osteoactivin Protein, Mouse (HEK293, His)

GPNMB/bone active protein shows promise as a melanogenic enzyme, possibly involved in melanin synthesis. Its regulatory influence on melanin production highlights its critical role in pigment formation. GPNMB/Osteoactivin Protein, Mouse (HEK293, His) is the recombinant mouse-derived GPNMB/Osteoactivin protein, expressed by HEK293 , with C-His labeled tag.

The GPNMB/Osteoactivin protein emerges as a promising candidate for a melanogenic enzyme, suggesting its potential involvement in the synthesis of melanin. Recent research underscores the regulatory influence of GPNMB/Osteoactivin on melanogenesis, highlighting its pivotal role in the intricate process of pigment formation. The protein's capacity to modulate melanin production emphasizes its significance in unraveling the complexities of skin biology and pigmentation pathways. Investigating the molecular mechanisms governing GPNMB/Osteoactivin's role in melanogenesis holds promise for uncovering new insights into the control of skin pigmentation, offering potential avenues for innovative approaches to address disorders associated with pigmentation.

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 5× 10⁴ cells/well are added to GPNMB-coated plates (0.25 μg/mL and 100 μL/well), approximately >22.59% will adhere after 30 minutes at 37°C.

Mouse

HEK293

C-His

Q99P91 (K23-N502)

93695  [NCBI]

Extracellular Domain

KRFRDVLGHEQYPDHMREHNQLRGWSSDENEWDEHLYPVWRRGDGRWKDSWEGGRVQAVLTSDSPALVGSNITFVVNLVFPRCQKEDANGNIVYEKNCRNDLGLTSDLHVYNWTAGADDGDWEDGTSRSQHLRFPDRRPFPRPHGWKKWSFVYVFHTLGQYFQKLGRCSARVSINTVNLTAGPQVMEVTVFRRYGRAYIPISKVKDVYVITDQIPVFVTMSQKNDRNLSDEIFLRDLPIVFDVLIHDPSHFLNDSAISYKWNFGDNTGLFVSNNHTLNHTYVLNGTFNLNLTVQTAVPGPCPPPSPSTPPPPSTPPSPPPSPLPTLSTPSPSLMPTGYKSMELSDISNENCRINRYGYFRATITIVEGILEVSIMQIADVPMPTPQPANSLMDFTVTCKGATPMEACTIISDPTCQIAQNRVCSPVAVDGLCLLSVRRAFNGSGTYCVNFTLGDDASLALTSTLISIPGKDPDSPLRAVN

Approximately 96 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.