2. Epigenetics
  3. Protein Arginine Deiminase
  4. Adargiminase

Adargiminase (NEI-01) is a modified arginine-depleting enzyme and albumin binder. Adargiminase catalyzes the conversion of arginine to citrulline and ammonia, reduces plasma arginine levels to undetectable levels, and binds to serum albumin from Mus musculus (mouse), Rattus norvegicus (rat), Canis lupus familiaris (dog) and Homo sapiens (human) to extend its half-life. Adargiminase inhibits the viability of ASS1-negative pancreatic cancer cells, and reduces tumor volume and weight. Adargiminase can be used for the research of pancreatic cancer.

For research use only. We do not sell to patients.

Adargiminase

Adargiminase Chemical Structure

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Adargiminase Related Antibodies

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Description

Adargiminase (NEI-01) is a modified arginine-depleting enzyme and albumin binder. Adargiminase catalyzes the conversion of arginine to citrulline and ammonia, reduces plasma arginine levels to undetectable levels, and binds to serum albumin from Mus musculus (mouse), Rattus norvegicus (rat), Canis lupus familiaris (dog) and Homo sapiens (human) to extend its half-life. Adargiminase inhibits the viability of ASS1-negative pancreatic cancer cells, and reduces tumor volume and weight. Adargiminase can be used for the research of pancreatic cancer[1].

In Vitro

Adargiminase (0.001-10 μg/mL; 72 h) potently inhibits the viability of ASS1-negative MIA PaCa-2 and PANC-1 pancreatic cancer cells, but exerts no inhibitory effect on ASS1-positive SMMC7721 hepatocellular carcinoma cells[1].
Adargiminase (0.1 μg/mL; 6-96 h) induces activation of the autophagy pathway (increased levels of LC3-II, Beclin 1, and phosphorylated AMPKα) and activation of the apoptosis pathway (decreased level of PARP-1) in MIA PaCa-2 pancreatic cancer cells[1].
Adargiminase (0.001-1 μg/mL; 72 h) has no effect on NEI-01-adapted MIA PaCa-2 pancreatic cancer cells, but resensitizes these cells after 96 h of culture in NEI-01-free medium[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Adargiminase Related Antibodies

Cell Viability Assay[1]

Cell Line: Human pancreatic cancer cell lines (MIA PaCa-2, PANC-1), human hepatocellular carcinoma cell line (SMMC7721)
Concentration: 0.001-10 μg/mL
Incubation Time: 72 h
Result: Inhibited viability of ASS1-negative MIA PaCa-2 cells with an IC50 of 0.086 μg/mL.
Inhibited viability of ASS1-negative PANC-1 cells with an IC50 of 0.190 μg/mL.
Showed no significant inhibition of ASS1-positive SMMC7721 cells, with an IC50 >10 μg/mL.

Western Blot Analysis[1]

Cell Line: MIA PaCa-2 human pancreatic cancer cell line
Concentration: 0.1 μg/mL
Incubation Time: 6-96 h
Result: Increased levels of LC3-II, Beclin 1, and phospho-AMPKα.
Decreased levels of PARP-1.
Indicated activation of both autophagic and apoptotic pathways.

Cell Viability Assay[1]

Cell Line: NEI-01-adapted and washout MIA PaCa-2 human pancreatic cancer cell line
Concentration: 0.001-1 μg/mL
Incubation Time: 72 h
Result: Showed resistance to NEI-01 treatment in NEI-01-adapted MIA PaCa-2 cells.
Resensitized washout MIA PaCa-2 cells to NEI-01 with an IC50 of 0.012 μg/mL.
In Vivo

NEI-01 (Adargiminase) (2-5 U; i.p.; twice a week) significantly reduces pancreatic tumor volume and weight in a mouse xenograft model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c female athymic nude mice (4-6-week-old; pancreatic cancer xenograft model)[1]
Dosage: 2 U; 5 U
Administration: i.p.; twice a week
Result: Significantly reduced tumor volume compared to control (2 U dose).
Significantly reduced tumor volume (p < 0.01) and tumor weight (p = 0.004) compared to control (5 U dose).
Reduced plasma arginine levels over the course of the experiment.
Induced only a very slight increase in ASS1 expression in tumor tissue.
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

[Adargiminase]
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Adargiminase Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AdargiminaseNEI-01NEI01NEI 01Protein Arginine DeiminasePeptidylarginine Deiminasehuman pancreatic mouse xenograft modelsdog serum albuminrat serum albuminASS1-negative pancreatic cancer cellsmouse serum albuminMIA PaCa-2 pancreatic cancer cellsautophagic pathwaysASS1-positive SMMC7721 hepatocellular carcinoma cellsapoptotic pathwaysPANC-1 pancreatic cancer cellsInhibitorinhibitorinhibit

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