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Art. -Nr.: HY-P992098

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Adargiminase

(Synonyms: NEI-01)

Adargiminase (NEI-01) is a modified arginine-depleting enzyme and albumin binder. Adargiminase catalyzes the conversion of arginine to citrulline and ammonia, reduces plasma arginine levels to undetectable levels, and binds to serum albumin from Mus musculus (mouse), Rattus norvegicus (rat), Canis lupus familiaris (dog) and Homo sapiens (human) to extend its half-life. Adargiminase inhibits the viability of ASS1-negative pancreatic cancer cells, and reduces tumor volume and weight. Adargiminase can be used for the research of pancreatic cancer.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Adargiminase Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
- Conjugated
- Reconsititution
- Format
- Anwendung
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Adargiminase (0.001-10 μg/mL; 72 h) potently inhibits the viability of ASS1-negative MIA PaCa-2 and PANC-1 pancreatic cancer cells, but exerts no inhibitory effect on ASS1-positive SMMC7721 hepatocellular carcinoma cells^[1].
Adargiminase (0.1 μg/mL; 6-96 h) induces activation of the autophagy pathway (increased levels of LC3-II, Beclin 1, and phosphorylated AMPKα) and activation of the apoptosis pathway (decreased level of PARP-1) in MIA PaCa-2 pancreatic cancer cells^[1].
Adargiminase (0.001-1 μg/mL; 72 h) has no effect on NEI-01-adapted MIA PaCa-2 pancreatic cancer cells, but resensitizes these cells after 96 h of culture in NEI-01-free medium^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Human pancreatic cancer cell lines (MIA PaCa-2, PANC-1), human hepatocellular carcinoma cell line (SMMC7721)
Concentration:

0.001-10 μg/mL
Incubation Time:

72 h
Result:

Inhibited viability of ASS1-negative MIA PaCa-2 cells with an IC₅₀ of 0.086 μg/mL.
Inhibited viability of ASS1-negative PANC-1 cells with an IC₅₀ of 0.190 μg/mL.
Showed no significant inhibition of ASS1-positive SMMC7721 cells, with an IC₅₀ >10 μg/mL.

Western Blot Analysis^[1]

Cell Line:

MIA PaCa-2 human pancreatic cancer cell line
Concentration:

0.1 μg/mL
Incubation Time:

6-96 h
Result:

Increased levels of LC3-II, Beclin 1, and phospho-AMPKα.
Decreased levels of PARP-1.
Indicated activation of both autophagic and apoptotic pathways.

Cell Viability Assay^[1]

Cell Line:

NEI-01-adapted and washout MIA PaCa-2 human pancreatic cancer cell line
Concentration:

0.001-1 μg/mL
Incubation Time:

72 h
Result:

Showed resistance to NEI-01 treatment in NEI-01-adapted MIA PaCa-2 cells.
Resensitized washout MIA PaCa-2 cells to NEI-01 with an IC₅₀ of 0.012 μg/mL.

In Vivo

NEI-01 (Adargiminase) (2-5 U; i.p.; twice a week) significantly reduces pancreatic tumor volume and weight in a mouse xenograft model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c female athymic nude mice (4-6-week-old; pancreatic cancer xenograft model)^[1]
Dosage:

2 U; 5 U
Administration:

i.p.; twice a week
Result:

Significantly reduced tumor volume compared to control (2 U dose).
Significantly reduced tumor volume (p < 0.01) and tumor weight (p = 0.004) compared to control (5 U dose).
Reduced plasma arginine levels over the course of the experiment.
Induced only a very slight increase in ASS1 expression in tumor tissue.

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Format

Product Image

Anwendung

ELISA, FACS, Functional assay

Chemical Information

SMILES

[Adargiminase]
Synonyms

NEI-01
Formulation

Please refer to the lot-specific COA for specific buffer information.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Inhibitory Antibodies User Guide (603 KB)

Verweise

[1]. Chow JPH, et al. A modified arginine-depleting enzyme NEI-01 inhibits growth of pancreatic cancer cells. PLoS One. 2020;15(4):e0231633. Published 2020 Apr 30. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
- Conjugated
- Reconsititution
- Format
- Anwendung
Chemical Information
Reinheit & Dokumentation
Verweise

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

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CAS. Nr.:
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Reinheit:
Menge:

Adargiminase

Cell Counting Kit-8

PBS Buffer (1×)

DPBS Buffer (1×)

Cytotoxicity LDH Assay Kit