DDR1 Protein, Human (Active, sf9, His-GST)
The DDR1 protein is a tyrosine kinase and cell surface receptor for fibrillar collagen that tightly regulates cell attachment and affects extracellular matrix processes. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC, activating MAP kinase. DDR1 Protein, Human (sf9, His-GST) is the recombinant human-derived DDR1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The DDR1 protein is a tyrosine kinase and cell surface receptor for fibrillar collagen that tightly regulates cell attachment and affects extracellular matrix processes. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC, activating MAP kinase. DDR1 Protein, Human (sf9, His-GST) is the recombinant human-derived DDR1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
DDR1 protein serves as a tyrosine kinase and functions as a cell surface receptor for fibrillar collagen, playing a pivotal role in regulating cell attachment to the extracellular matrix and influencing processes such as extracellular matrix remodeling, cell migration, differentiation, survival, and proliferation. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC and activates MAP kinases. This activation, in turn, leads to the up-regulation of matrix metalloproteinases MMP2, MMP7, and MMP9, facilitating extracellular matrix remodeling and promoting cell migration and wound healing. DDR1's significance extends to various physiological processes, including normal blastocyst implantation during pregnancy, mammary gland differentiation, lactation, and the maintenance of ear morphology and hearing. Additionally, DDR1 contributes to arterial wound healing by promoting smooth muscle cell migration and plays a role in tumor cell invasion, further underscoring its diverse functional repertoire. Notably, DDR1 phosphorylates PTPN11 as part of its regulatory activities.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
Q08345-1 (R444-V913)
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Molecular Construction
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N-term
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His-GST
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DDR1 (R444-V913)
Accession # Q08345-1 -
C-term
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Protein Length
Partial
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Synonyms
Epithelial discoidin domain-containing receptor 1; HGK2; CD167a; CAK; EDDR1
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AA Sequence
RRLLSKAERRVLEEELTVHLSVPGDTILINNRPGPREPPPYQEPRPRGNPPHSAPCVPNGSALLLSNPAYRLLLATYARPPRGPGPPTPAWAKPTNTQAYSGDYMEPEKPGAPLLPPPPQNSVPHYAEADIVTLQGVTGGNTYAVPALPPGAVGDGPPRVDFPRSRLRFKEKLGEGQFGEVHLCEVDSPQDLVSLDFPLNVRKGHPLLVAVKILRPDATKNARNDFLKEVKIMSRLKDPNIIRLLGVCVQDDPLCMITDYMENGDLNQFLSAHQLEDKAAEGAPGDGQAAQGPTISYPMLLHVAAQIASGMRYLATLNFVHRDLATRNCLVGENFTIKIADFGMSRNLYAGDYYRVQGRAVLPIRWMAWECILMGKFTTASDVWAFGVTLWEVLMLCRAQPFGQLTDEQVIENAGEFFRDQGRQVYLSRPPACPQGLYELMLRCWSRESEQRPPFSQLHRFLAEDALNTV
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Predicted Molecular Mass
80 kDa
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Molecular Weight
Approximately 80 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 3 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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