Protocol for detecting lysosome quantity and membrane permeability in acute myeloid leukemia cell lines
- STAR Protoc. 2024 Sep 20;5(3):103309. doi: 10.1016/j.xpro.2024.103309.
Affiliations
- 1. Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China. Electronic address: [email protected].
- 2. Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China; Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
- 3. Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China. Electronic address: [email protected].
- 4. Department of Hematology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510180, China. Electronic address: [email protected].
PMID: 39269898
DOI: 10.1016/j.xpro.2024.103309
Abstract
Lysosomal function and activity are essential to support cellular adaptation to multiple stresses. For example, certain drugs can induce increased lysosomal membrane permeability to exert their anti-cancer effects. Here, we present a protocol to evaluate the lysosome alterations induced by drug treatment. We first describe the steps for inducing lysosomal alterations in cultured cells. We then show how to quantify the number of lysosomes, assess the integrity of lysosomal membranes, and determine lysosomal membrane permeabilization by using Galectin puncta assay. For complete details on the use and execution of this protocol, please refer to Jiang et al.1.
Keywords
cancer; cell biology; cell culture; flow cytometry.
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