RSV604 racemate
Based on 1 Customer Validation
RSV604 (A-60444) racemate is a racemic mixture, shows less potency against strains of respiratory syncytial virus (RSV) than the S-isomer.
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- Pureté: 98.60%
- CAS No.: 676128-62-4
- Formule: C22H17FN4O2
- Masse moléculaire:388.39
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEp-2 | IC50 |
0.75 μM
Compound: 42
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Antiviral activity against RSV B in Hep2 cells by plaque assay
Antiviral activity against RSV B in Hep2 cells by plaque assay
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[PMID: 16570927] |
| HEp-2 | IC50 |
1.9 μM
Compound: 42
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Antiviral activity against RSV B in Hep2 cells by ELISA
Antiviral activity against RSV B in Hep2 cells by ELISA
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[PMID: 16570927] |
| HEp-2 | IC50 |
2.2 μM
Compound: 42
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Antiviral activity against RSV Long in Hep2 cells by plaque assay
Antiviral activity against RSV Long in Hep2 cells by plaque assay
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[PMID: 16570927] |
| HEp-2 | IC50 |
2.5 μM
Compound: 42
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Antiviral activity against RSV Long in Hep2 cells by ELISA
Antiviral activity against RSV Long in Hep2 cells by ELISA
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[PMID: 16570927] |
| HEp-2 | IC50 |
2.7 μM
Compound: 42
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Antiviral activity against RSV RSS in Hep2 cells by ELISA
Antiviral activity against RSV RSS in Hep2 cells by ELISA
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[PMID: 16570927] |
| HEp-2 | IC50 |
2.8 μM
Compound: 42
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Antiviral activity against RSV A2 in Hep2 cells by plaque assay
Antiviral activity against RSV A2 in Hep2 cells by plaque assay
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[PMID: 16570927] |
| HEp-2 | IC50 |
27.8 μM
Compound: 17a
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Antiviral activity against RSV RSS in Hep2 cells by XTT assay
Antiviral activity against RSV RSS in Hep2 cells by XTT assay
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[PMID: 17341059] |
| HEp-2 | IC50 |
3 μM
Compound: 42
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Antiviral activity against RSV RSS in Hep2 cells by plaque assay
Antiviral activity against RSV RSS in Hep2 cells by plaque assay
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[PMID: 16570927] |
| HEp-2 | IC50 |
3.3 μM
Compound: 42
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Antiviral activity against RSV RSS in Hep2 cells by whole cell XTT assay
Antiviral activity against RSV RSS in Hep2 cells by whole cell XTT assay
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[PMID: 16570927] |
Chemical Information
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CAS No. 676128-62-4
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Appearance Solid
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Masse moléculaire 388.39
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Formule C22H17FN4O2
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Color White to off-white
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SMILES
O=C1C(NC(NC2=CC=CC=C2F)=O)N=C(C3=CC=CC=C3)C4=CC=CC=C4N1
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Synonyms
A-60444 racemate
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvant et solubilité
DMSO : 50 mg/mL (128.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (7.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5747 mL | 12.8737 mL | 25.7473 mL | 64.3683 mL |
| 5 mM | 0.5149 mL | 2.5747 mL | 5.1495 mL | 12.8737 mL | |
| 10 mM | 0.2575 mL | 1.2874 mL | 2.5747 mL | 6.4368 mL | |
| 15 mM | 0.1716 mL | 0.8582 mL | 1.7165 mL | 4.2912 mL | |
| 20 mM | 0.1287 mL | 0.6437 mL | 1.2874 mL | 3.2184 mL | |
| 25 mM | 0.1030 mL | 0.5149 mL | 1.0299 mL | 2.5747 mL | |
| 30 mM | 0.0858 mL | 0.4291 mL | 0.8582 mL | 2.1456 mL | |
| 40 mM | 0.0644 mL | 0.3218 mL | 0.6437 mL | 1.6092 mL | |
| 50 mM | 0.0515 mL | 0.2575 mL | 0.5149 mL | 1.2874 mL | |
| 60 mM | 0.0429 mL | 0.2146 mL | 0.4291 mL | 1.0728 mL | |
| 80 mM | 0.0322 mL | 0.1609 mL | 0.3218 mL | 0.8046 mL | |
| 100 mM | 0.0257 mL | 0.1287 mL | 0.2575 mL | 0.6437 mL |
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