Bi-27 Serum-free Supplement (50×), Antioxidant-Free
MCE Bi-27 Serum-free Supplement (50×), Antioxidant-Free is a customized neuronal culture supplement developed by removing antioxidants components (such as vitamins, superoxide dismutase, catalase, etc.) from the standard Bi-27 formulation. It is specifically designed for investigating the roles of reactive oxygen species (ROS) in aging, cytotoxicity, apoptosis, and chronic neurological diseases. This formulation provides a stable culture environment for mechanistic studies without the interference of exogenous antioxidants.
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Storage :
-20°C, 2 years
Keep away from light
Shipping with dry ice
- Manual (1297KB)
- COA
Description & Advantages
MCE Bi-27 Serum-free Supplement (50×), Antioxidant-Free is a customized neuronal culture supplement developed by removing antioxidants components (such as vitamins, superoxide dismutase, catalase, etc.) from the standard Bi-27 formulation. It is specifically designed for investigating the roles of reactive oxygen species (ROS) in aging, cytotoxicity, apoptosis, and chronic neurological diseases. This formulation provides a stable culture environment for mechanistic studies without the interference of exogenous antioxidants.
The recommended working concentration of Bi-27 Supplement is 2% (v/v), corresponding to a 1:50 dilution before use in the culture medium.
Livraison
Shipping with dry ice.
Protocole
Prepare complete Neuronal basal Medium
1. Thaw MCE Bi-27 Serum-free Supplement (50×), Antioxidant -Free overnight at 4°C.
2. Aseptically add 2% Bi-27 and 0.5mM L-Glutamine to the Neuronal Basal Medium before use.
3. Primary Hippocampal Neuron Culture: Add 25 µM of L-glutamate to the complete Neuronal Basal Medium. The medium can be replaced after 4 days of culture, and glutamate should not be added to the medium after this change.
Note: Once prepared, the complete Neuronal Basal Medium can be stored at 4°C for two weeks, protected from light.
Stockage
-20°C, 2 years
Keep away from light
Shipping with dry ice
Attention
1. This product appears as a light yellow liquid. The color may not be consistent following freezing, but it can fully dissolve in a 37°C water bath.
2. It is recommended to appropriately aliquot the solution to avoid repeat freeze-thaw cycles.
3. Pay attention to aseptic operation to avoid contamination.
4. This product is for R&D use only, not for drug, household, or other uses.
5. For your safety and health, please wear a lab coat and disposable gloves to operate.
Composants
| Components | HY-K3014C-10 mL | HY-K3014C-50 mL |
|---|---|---|
| Bi-27 Serum-free Supplement (50×), Antioxidant-Free | 10 mL | 10 mL × 5 |
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