Cat. No.: HY-N0171B

Beta-Sitosterol (purity>75%)

(Synonyms: β-Sitosterol (purity>75%); 22,23-Dihydrostigmasterol (purity>75%))

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Beta-Sitosterol (purity>75%) is a phytosterol with oral activity. Beta-Sitosterol (purity>75%) interferes with a variety of cell signaling pathways, including the cell cycle, apoptosis and cell proliferation. Beta-Sitosterol (purity>75%) has anti-inflammatory, antioxidant, and antitumor activities.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 83-46-5
Formula: C₂₉H₅₀O
Molecular Weight:414.71

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Beta-Sitosterol (purity>75%) Chemical Structure

CAS No.: 83-46-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
1A9	ED₅₀	10.6 μg/mL Compound: 17	Cytotoxicity against human 1A9 cells after 6 days by SRB assay Cytotoxicity against human 1A9 cells after 6 days by SRB assay	[PMID: 14640511]
1A9	ED₅₀	16.8 μg/mL Compound: 17	Cytotoxicity against human 1A9 cells after 3 days by SRB assay Cytotoxicity against human 1A9 cells after 3 days by SRB assay	[PMID: 14640511]
1A9/ptx-10	ED₅₀	20 μg/mL Compound: 17	Cytotoxicity against human 1A9/PTX10 cells after 3 days by SRB assay Cytotoxicity against human 1A9/PTX10 cells after 3 days by SRB assay	[PMID: 14640511]
1A9/ptx-10	ED₅₀	9.5 μg/mL Compound: 17	Cytotoxicity against human 1A9/PTX10 cells after 6 days by SRB assay Cytotoxicity against human 1A9/PTX10 cells after 6 days by SRB assay	[PMID: 14640511]
A2780	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human A2780 cells after 96 hrs by MTT assay Cytotoxicity against human A2780 cells after 96 hrs by MTT assay	[PMID: 17125236]
A549	ED₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human A549 cells after 3 days by SRB assay Cytotoxicity against human A549 cells after 3 days by SRB assay	[PMID: 14640511]
A549	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human A549 cells after 96 hrs by MTT assay Cytotoxicity against human A549 cells after 96 hrs by MTT assay	[PMID: 17125236]
Bel-7402	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay Cytotoxicity against human Bel-7402 cells after 96 hrs by MTT assay	[PMID: 17125236]
BGC-823	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay Cytotoxicity against human BGC-823 cells after 96 hrs by MTT assay	[PMID: 17125236]
ECV-304	IC₅₀	472 μM Compound: beta-sitosterol	Membranolytic activity in human ECV304 cells assessed as leakage of intracellular lactate dehydrogenase after 2 hrs by spectrophotometry Membranolytic activity in human ECV304 cells assessed as leakage of intracellular lactate dehydrogenase after 2 hrs by spectrophotometry	[PMID: 22503361]
ECV-304	IC₅₀	472 μM Compound: Beta-sitosterol	Membrane toxicity against human ECV304 cells after 2 hrs by LDH release assay Membrane toxicity against human ECV304 cells after 2 hrs by LDH release assay	[PMID: 24084294]
ECV-304	IC₅₀	61 μM Compound: beta-sitosterol	Cytotoxicity against human ECV304 cells after 72 hrs by Hoechst 33258 staining based fluorescence assay Cytotoxicity against human ECV304 cells after 72 hrs by Hoechst 33258 staining based fluorescence assay	[PMID: 22503361]
ECV-304	IC₅₀	61 μM Compound: Beta-sitosterol	Cytotoxicity against human ECV304 cells after 72 hrs by MTT assay Cytotoxicity against human ECV304 cells after 72 hrs by MTT assay	[PMID: 24084294]
HCT-8	ED₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human HCT8 cells after 3 days by SRB assay Cytotoxicity against human HCT8 cells after 3 days by SRB assay	[PMID: 14640511]
HCT-8	IC₅₀	>10 μg/mL Compound: page 1629, R26C1	Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay	[PMID: 17125236]
HeLa	IC₅₀	46.22 μM Compound: 9	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19447618]
HEp-2	IC₅₀	11.4 μM Compound: 119	Antiproliferative activity against human Hep2 cells by MTT assay Antiproliferative activity against human Hep2 cells by MTT assay	[PMID: 30830783]
HT-1080	IC₅₀	2.5 μM Compound: Angelicin	Cytotoxicity against human HT1080 cells assessed as reduction in cell viability Cytotoxicity against human HT1080 cells assessed as reduction in cell viability	[PMID: 30660827]
HUVEC	ED₅₀	>5 μg/mL Compound: beta-sitosterol	Cytotoxicity against HUVEC Cytotoxicity against HUVEC	[PMID: 15043409]
J774	IC₅₀	>241.1 μM Compound: 11	Cytotoxicity against mouse J774 cells by alamar blue assay Cytotoxicity against mouse J774 cells by alamar blue assay	[PMID: 17637068]
KB	ED₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human KB cells after 3 days by SRB assay Cytotoxicity against human KB cells after 3 days by SRB assay	[PMID: 14640511]
KB	IC₅₀	>10 μg/mL Compound: 119	Antiproliferative activity against human KB cells assessed as reduction in cell viability Antiproliferative activity against human KB cells assessed as reduction in cell viability	[PMID: 30830783]
KB	IC₅₀	>241.5 μM Compound: 119	Antiproliferative activity against human KB/HeLa cells assessed as reduction in cell viability Antiproliferative activity against human KB/HeLa cells assessed as reduction in cell viability	[PMID: 30830783]
LNCaP	ED₅₀	>5 μg/mL Compound: beta-sitosterol	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 15043409]
Lu1	ED₅₀	>5 μg/mL Compound: beta-sitosterol	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 15043409]
MCF7	ED₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human MCF7 cells after 3 days by SRB assay Cytotoxicity against human MCF7 cells after 3 days by SRB assay	[PMID: 14640511]
MCF7	ED₅₀	>5 μg/mL Compound: beta-sitosterol	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 15043409]
MCF7	IC₅₀	42.1 μM Compound: 9	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 19447618]
PC-3	ED₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human PC3 cells after 3 days by SRB assay Cytotoxicity against human PC3 cells after 3 days by SRB assay	[PMID: 14640511]
SK-MEL-1	IC₅₀	>50 μM Compound: 9	Cytotoxicity against human SK-MEL-1 cells by MTT assay Cytotoxicity against human SK-MEL-1 cells by MTT assay	[PMID: 19447618]
U-87MG ATCC	ED₅₀	>20 μg/mL Compound: 17	Cytotoxicity against human U87MG cells after 3 days by SRB assay Cytotoxicity against human U87MG cells after 3 days by SRB assay	[PMID: 14640511]
Vero	IC₅₀	>128 μg/mL Compound: 4	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 18818073]

In Vitro

Beta-Sitosterol purity>75% (16 μM, 1, 3, 5 days) can inhibit the growth of MDA-MB-231 human breast cancer cells and induce cell apoptosis^[1].
Beta-Sitosterol purity>75% (120, 240 μM, 24 h) shows significant dose-dependent growth inhibition on COLO 320 DM cells (IC₅₀ 266.2 μM). The expression of β-catenin and PCNA antigens in human colon cancer cells was inhibited by scavenging reactive oxygen species to induce cell apoptosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

MDA-MB-231
Concentration:

16 μM
Incubation Time:

3 days
Result:

No cytotoxicity at 16 μM

Apoptosis Analysis^[1]

Cell Line:

MDA-MB-231
Concentration:

16 μM
Incubation Time:

5 days
Result:

Increased 33% apoptosis when assayed using 1×10⁴ cells and a 6-fold increase in apoptosis when assayed using a smaller number of cells 5×10³.

Western Blot Analysis^[2]

Cell Line:

COLO 320 DM
Concentration:

15, 30, 60, 120 μM
Incubation Time:

24 h
Result:

Decreased β-catenin and PCNA expression.

In Vivo

Beta-Sitosterol purity>75% (10-20 mg/kg, suspended in 0.1% CMC, orally, once a day for 16 weeks) can prevent cancer in the rat model of colon cancer^[2].
Beta-Sitosterol purity>75% (10, 15, 20 mg/kg, orally, for 21 consecutive days) has anti-hyperglycemic and antioxidant effects in streptozotocin (HY-13753) induced experimental hyperglycemic rat models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Colon cancer rats model ^[2]
Dosage:

10, 20 mg/kg
Administration:

p.o., suspended in 0.1% CMC (1.0 mL)
Result:

Reduced the number of aberrant crypt and crypt multiplicity in a dose-dependent manner.

Animal Model:

Streptozotocin-induced hyperglycemia rats model^[3]
Dosage:

10, 15, 20 mg/kg
Administration:

p.o.
Result:

Increased insulin levels and decreased HbA1c levels.
Improved pancreatic antioxidant levels and decreased LPO levels.

Chemical Information

CAS No. 83-46-5
Appearance Solid
Molecular Weight 414.71
Formula C₂₉H₅₀O
Color White to off-white
SMILES

CC[C@@H](C(C)C)CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Synonyms

β-Sitosterol (purity>75%); 22,23-Dihydrostigmasterol (purity>75%)
Structure Classification
- Steroids
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMF : 16.67 mg/mL (40.20 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4113 mL

12.0566 mL

24.1132 mL

5 mM

0.4823 mL

2.4113 mL

4.8226 mL

10 mM

0.2411 mL

1.2057 mL

2.4113 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMF 90% Corn Oil

Solubility: ≥ 1.67 mg/mL (4.03 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Awad AB, et al. Inhibition of growth and stimulation of apoptosis by beta-sitosterol treatment of MDA-MB-231 human breast cancer cells in culture. Int J Mol Med. 2000 May;5(5):541-5. [Content Brief]

[2]. Baskar AA, et al. Chemopreventive potential of beta-Sitosterol in experimental colon cancer model--an in vitro and In vivo study. BMC Complement Altern Med. 2010 Jun 4;10:24. [Content Brief]

[3]. Gupta R, et al. Antidiabetic and antioxidant potential of β-sitosterol in streptozotocin-induced experimental hyperglycemia. J Diabetes. 2011 Mar;3(1):29-37. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMF	1 mM	2.4113 mL	12.0566 mL	24.1132 mL	60.2831 mL
	5 mM	0.4823 mL	2.4113 mL	4.8226 mL	12.0566 mL
	10 mM	0.2411 mL	1.2057 mL	2.4113 mL	6.0283 mL
	15 mM	0.1608 mL	0.8038 mL	1.6075 mL	4.0189 mL
	20 mM	0.1206 mL	0.6028 mL	1.2057 mL	3.0142 mL
	25 mM	0.0965 mL	0.4823 mL	0.9645 mL	2.4113 mL
	30 mM	0.0804 mL	0.4019 mL	0.8038 mL	2.0094 mL
	40 mM	0.0603 mL	0.3014 mL	0.6028 mL	1.5071 mL

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Beta-Sitosterol (purity>75%)

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Beta-Sitosterol (purity>75%) Related Classifications

Cat. No.: HY-N0171B