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DDR2 Protein, Rhesus Macaque (HEK293, His)

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Based on 1 Customer Validation

The DDR2 protein is a tyrosine kinase critical in tissue remodeling, acting as a cell surface receptor for fibrillar collagen and affecting cell differentiation, extracellular matrix remodeling, migration, and proliferation. DDR2 is critical for bone development and centrally regulates osteoblast differentiation and chondrocyte maturation through MAP kinase signaling and activation of RUNX2. DDR2 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived DDR2 protein, expressed by HEK293 , with C-His labeled tag.

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  • Species: Rhesus Macaque
  • Source: HEK293
  • Almacenamiento:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
  • DDR2 Protein, Rhesus Macaque (HEK293, His) Product Image
  • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
  • DDR2 Protein, Rhesus Macaque (HEK293, His) Product Image
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Encuentre su distribuidor local Your local distributor: Hycultec GmbHHycultec
  • Actividad biológica
  • Technical Parameters
  • Product Properties
  • Documentación
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Actividad biológica

Descripciòn

The DDR2 protein is a tyrosine kinase critical in tissue remodeling, acting as a cell surface receptor for fibrillar collagen and affecting cell differentiation, extracellular matrix remodeling, migration, and proliferation. DDR2 is critical for bone development and centrally regulates osteoblast differentiation and chondrocyte maturation through MAP kinase signaling and activation of RUNX2. DDR2 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived DDR2 protein, expressed by HEK293 , with C-His labeled tag.

Background

DDR2 protein, a tyrosine kinase integral to the regulation of tissue remodeling, functions as a cell surface receptor for fibrillar collagen, exerting control over cell differentiation, extracellular matrix remodeling, cell migration, and proliferation. Crucial for normal bone development, DDR2 plays a central role in osteoblast differentiation and chondrocyte maturation through a signaling pathway involving MAP kinases, culminating in the activation of the transcription factor RUNX2. Additionally, DDR2 regulates extracellular matrix remodeling by up-regulating collagenases MMP1, MMP2, and MMP13, thereby facilitating cell migration and tumor cell invasion. Its involvement extends to promoting fibroblast migration and proliferation, contributing to the dynamic process of cutaneous wound healing.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL can bind Rhesus Macaque DDR2 with an apparent KD is 7.846 nM.

Results
  • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
    Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL can bind Rhesus Macaque DDR2 with an apparent KD is 7.846 nM.

Technical Parameters

  • Species Rhesus Macaque
  • Source HEK293
  • Tag C-6*His
  • Accession

    XP_001118219.1 (Q24-R399)

  • Gene ID
    720124  [NCBI]
  • Molecular Construction
    • N-term
    • DDR2 (Q24-R399)
      Accession # XP_001118219.1
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    Discoidin domain-containing receptor 2; CD167b; Ddr2; Ntrkr3; Tkt; Tyro10

  • AA Sequence

    QVNPAICRYPLGMSGGQIPDEDITASSQWSESTAAKYGRLDSEEGDGAWCPEIPVEPDDLKEFLQIDLHTLHFITLVGTQGRHAGGHGIEFAPMYKINYSRDGTRWISWRNRHGKQVLDGNSNPYDIFLKDLEPPIVARFVRFIPVTDHSMNVCMRVELYGCVWLDGLVSYNAPAGQQFVLPGGSIIYLNDSVYDGAVGYSMTEGLGQLTDGVSGLDDFTQTHEYHVWPGYDYVGWRNESATNGYIEIMFEFDRIRNFTTMKVHCNNMFAKGVKIFKEVQCYFRSEASEWEPNAISFPLVLDDVNPSARFVTVPLHHRMASAIKCQYHFADTWMMFSEITFQSDAAMYNNSGALPTSPMTPTTYDPMLKIDDSNTR

  • Peso molecular

    Approximately 50-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Pureza

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Envío

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Calculadora de dilución

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL

DDR2 Protein, Rhesus Macaque (HEK293, His) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL can bind Rhesus Macaque DDR2 with an apparent KD is 7.846 nM.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL can bind Rhesus Macaque DDR2 with an apparent KD is 7.846 nM.

  • DDR2 Protein, Rhesus Macaque (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
  • Experimental Validation Results for DDR2 Protein, Rhesus Macaque (HEK293, His)
  • DDR2 Protein, Rhesus Macaque (HEK293, His) Product Image