BeRST voltage sensitive dye
BeRST voltage sensitive dye is a silicon-rhodamine-based voltage-sensitive dye that serves as a membrane potential indicator, featuring fast response kinetics, high sensitivity, photostability, and excellent signal-to-noise ratio. BeRST voltage sensitive dye relies on the photoinduced electron transfer (PeT) mechanism to report changes in neuronal membrane potential via fluorescent signals. BeRST voltage sensitive dye localizes to the extracellular surface of neuronal plasma membranes, including cell bodies and neurites. BeRST voltage sensitive dye can be used in studies related to Alzheimer's disease (Ex/Em = 658/683 nm).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C45H51N3O4SSi
- 分子量:758.05
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
BeRST voltage sensitive dye (BeRST 1) (0.1-1 µM; 20 min loading, 7 days chronic amyloid beta exposure) enables high-fidelity, high-throughput optical recording of action potentials and network connectivity in dissociated rat hippocampal neurons, with near-perfect correlation to patch-clamp electrophysiology, and reliably detects changes in neuronal activity and connectivity following pharmacological inhibition, chronic amyloid beta 1-42 exposure, and developmental maturation[1].
BeRST voltage sensitive dye (500 nM; 30 min) enables high-temporal-resolution voltage imaging of dissociated 14-16 DIV rat hippocampal neurons, detecting action potentials in single trials and revealing that β-hydroxybutyrate treatment significantly reduces neuronal firing frequency, particularly in cells with lower intrinsic firing rates[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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分子量 758.05
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分子式 C45H51N3O4SSi
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SMILES
COC1=C(/C=C/C2=CC=C(/C=C/C3=CC=C(/C(C4=CC=C(N(C)C)C=C4[Si](C)(C)C)=C5C=C/C(C=C/5)=[N+](C)/C)C(S(=O)([O-])=O)=C3)C=C2)C=CC(N(C)C)=C1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Walker AS, et al. Optical Spike Detection and Connectivity Analysis With a Far-Red Voltage-Sensitive Fluorophore Reveals Changes to Network Connectivity in Development and Disease. Frontiers in neuroscience. 2021;15:643859. [Content Brief]
[2]. Kirk MJ, et al. Monitoring neuronal activity with voltage-sensitive fluorophores. Methods in enzymology. 2020;640:185-204. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
BeRST voltage sensitive dye 関連分類
HY-D3480 関連分類
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