NSP-DMAE-NHS
Based on 1 Customer Validation
NSP-DMAE-NHS is an acridinium ester-based signal amplifier that can act as a luminescent probe to effectively improve the detection performance of biosensors. NSP-DMAE-NHS preserves the biochemical activity of biomolecules during the conjugation process. NSP-DMAE-NHS has been successfully applied to label mouse anti-human PI3 monoclonal antibodies for chemiluminescent immunoassays, as well as for pre-competitive chemiluminescent immunochromatographic detection of TP53 fusion proteins. NSP-DMAE-NHS can be used in detection studies of cancer and related biomarkers.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.48%
- CAS 番号: 194357-64-7
- 分子式: C30H26N2O9S
- 分子量:590.60
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
NSP-DMAE-NHS (1 mg; 1 h shaking, 15 min quenching) successfully conjugates to anti-TP53 antibody, forming a chemiluminescent immunoconjugate with a minimum detectable sensitivity of 1.8 × 10-17 mol[1].
NSP-DMAE-NHS-based competitive chemiluminescent reagent strip assay (30 μL conjugates; 30 min sample-conjugate incubation, 30 min strip incubation) enables ultrasensitive, selective detection of TP53 fusion protein with a limit of detection of 0.0008 nng/mL (0.013 pM), a linear range of 0.001 to 100 ng/mL, and an IC50 of ~0.24 ng/mL[1].
NSP-DMAE-NHS-labeled anti-human PI3 monoclonal antibody supports a highly reliable, sensitive, and specific CLIA assay for PI3 quantification[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 194357-64-7
-
性状 Solid
-
分子量 590.60
-
分子式 C30H26N2O9S
-
Color Light yellow to green yellow
-
SMILES
O=C(C1=CC(C)=C(OC(C2=C3C=CC=CC3=[N+](CCCS(=O)([O-])=O)C4=C2C=CC=C4)=O)C(C)=C1)ON5C(CCC5=O)=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
溶剤 & 溶解度
DMSO : 10 mg/mL (16.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (273 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Wang L, et al. Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips. Appl Biochem Biotechnol. 2024;196(9):6315-6329. [Content Brief]
[2]. Xu M, et al. Systematic analysis of serum peptidase inhibitor 3 in psoriasis diagnosis and treatment. Clin Rheumatol. 2024;43(11):3361-3372. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6932 mL | 8.4660 mL | 16.9319 mL | 42.3298 mL |
| 5 mM | 0.3386 mL | 1.6932 mL | 3.3864 mL | 8.4660 mL | |
| 10 mM | 0.1693 mL | 0.8466 mL | 1.6932 mL | 4.2330 mL | |
| 15 mM | 0.1129 mL | 0.5644 mL | 1.1288 mL | 2.8220 mL |
Powered by Bioz