RIPK3 Protein, Mouse (P.pastoris, His)

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Based on 1 Customer Validation

RIPK3 Protein, participates in programmed necrosis (necroptosis) and apoptosis, contributing to diverse cellular processes. RIPK3 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived RIPK3 protein, expressed by P. pastoris , with N-6*His labeled tag.

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  • Species: Mouse
  • Source: P. pastoris
  • 保管条件:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • Help & FAQs

生物活性

製品説明

RIPK3 Protein, participates in programmed necrosis (necroptosis) and apoptosis, contributing to diverse cellular processes. RIPK3 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived RIPK3 protein, expressed by P. pastoris , with N-6*His labeled tag.

Background

RIPK3 (receptor-interacting serine/threonine-protein kinase 3), is involved in various cellular processes, including programmed necrosis (necroptosis) and apoptosis.

Verified Bioactivity

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Mouse
  • Source P. pastoris
  • Tag N-6*His
  • アクセッション番号
  • 遺伝子ID
  • Molecular Construction
    • N-term
    • 6*His
    • RIPK3 (M1-K486)
      Accession # Q9QZL0
    • C-term
  • Protein Length

    Full Length

  • 別名

    Ripk3; Rip3; Receptor-interacting serine/threonine-protein kinase 3; EC 2.7.11.1; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; mRIP3

  • AA Sequence

    MSSVKLWPTGASAVPLVSREELKKLEFVGKGGFGVVFRAHHRTWNHDVAVKIVNSKKISWEVKAMVNLRNENVLLLLGVTEDLQWDFVSGQALVTRFMENGSLAGLLQPECPRPWPLLCRLLQEVVLGMCYLHSLDPPLLHRDLKPSNILLDPELHAKLADFGLSTFQGGSQSGSGSGSGSRDSGGTLAYLDPELLFKVNLKASKASDVYSFGILVWAVLAGREAELVDKTSLIRETVCDRQSRPPLTELPPGSPETPGLEKLKELMIHCWGSQSENRPSFQDCEPKTNEVYNLVKDKVDAAVSEVKHYLSQHRSSGRNLSAREPSQRGTEMDCPRETMVSKMLDRLHLEEPSGPVPGKCPERQAQDTSVGPATPARTSSDPVAGTPQIPHTLPFRGTTPGPVFTETPGPHPQRNQGDGRHGTPWYPWTPPNPMTGPPALVFNNCSEVQIGNYNSLVAPPRTTASSSAKYDQAQFGRGRGWQPFHK

  • Predicted Molecular Mass

    54.8 kDa

  • 分子量

    Approximately 64 kDa, based on SDS-PAGE under reducing conditions.

  • 純度

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

性状

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

輸送条件

Room temperature in continental US; may vary elsewhere.

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)
=
Mass (in vial)
÷
Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始)
×
体積 (開始)
=
濃度 (終了)
×
体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50)
106 ÷
ng/mL