2. Academic Validation
  3. A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery

A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery

  • Dev Cell. 2012 Jul 17;23(1):166-80. doi: 10.1016/j.devcel.2012.04.019.
Andrea Burgo 1,  Véronique Proux-Gillardeaux,  Emmanuel Sotirakis,  Philippe Bun,  Alessandra Casano,  Agathe Verraes,  Ronald K H Liem,  Etienne Formstecher,  Maïté Coppey-Moisan,  Thierry Galli
Affiliations

Affiliation

  • 1 Institut Jacques Monod, UMR 7592, CNRS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France. [email protected]
PMID: 22705394 DOI: 10.1016/j.devcel.2012.04.019
Abstract

The compartmental organization of eukaryotic cells is maintained dynamically by vesicular trafficking. SNARE proteins play a crucial role in intracellular membrane fusion and need to be targeted to their proper donor or acceptor membrane. The molecular mechanisms that allow for the secretory vesicles carrying the v-SNARE TI-VAMP/VAMP7 to leave the cell center, load onto microtubules, and reach the periphery to mediate exocytosis are largely unknown. Here, we show that the TI-VAMP/VAMP7 partner Varp, a Rab21 guanine nucleotide exchange factor, interacts with GolginA4 and the Kinesin 1 Kif5A. Activated Rab21-GTP in turn binds to MACF1, an actin and microtubule regulator, which is itself a partner of GolginA4. These components are required for directed movement of TI-VAMP/VAMP7 vesicles from the cell center to the cell periphery. The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface.

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