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  6. TRAIL/TNFSF10 Protein, Rhesus Macaque

TRAIL/TNFSF10 Protein, Rhesus Macaque

Cat. No.: HY-P77256
COA Handling Instructions

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein mainly interacts with TRAIL-R1 and TRAIL-R2, and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. TRAIL Protein can recruit FADD and further recruit and activates caspase-8 after binding to DR4 or DR5. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell. TRAIL/TNFSF10 Protein, Rhesus Macaque is a recombinant Rhesus Macaque TRAIL (V114-G281) without any tag, which is expressed in E. coli.

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  • Biological Activity

  • Technical Parameters

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Description

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein mainly interacts with TRAIL-R1 and TRAIL-R2, and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. TRAIL Protein can recruit FADD and further recruit and activates caspase-8 after binding to DR4 or DR5. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways[1][2]. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell[1]. TRAIL/TNFSF10 Protein, Rhesus Macaque is a recombinant Rhesus Macaque TRAIL (V114-G281) without any tag, which is expressed in E. coli.

Background

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein is expressed in various tissues, especially in the spleen, lung, and prostate. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell. TRAIL proteins on NK and T cells is critical for controlling virus infections and tumor immune surveillance[1][2].
Human TRAIL consists of cytoplasmic domain (M1-V17), helical domain (L18-F38), and extracellular domain (T39-G281). Human TRAIL Protein shares < 70% common aa identity with mouse and rat. Mouse TRAIL Protein shares 86.94% common aa identity with rat.
TRAIL Protein mainly interacts with two agonistic TRAIL receptors (TRAIL-R1 and TRAIL-R2) and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. When binding to DR4 or DR5, TRAIL Protein can recruit FADD and further recruit and activates caspase-8. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways, such as NF-κB, PI3K/Akt, and MAPK pathway[1][2].
TRAIL induces apoptosis of tumor cells in a p53 independent manner. TRAIL-based therapies has high anti-tumor potential[3].

In Vitro

TRAIL (human, 1 ng/mL-1 μg/mL, 24 h) reduces cell viability of HepG2 cells[4].
TRAIL (human, 1-1 ng, 24 h) induces pathognomic features of asthma and stimulates the production of CCL2 in primary human bronchial epithelium cells[5].

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized rhesus macaque TNFSF10 at 10 μg/mL (100 μL/well) can bind rhesus macaque TNFRSF10D-Fc and the EC50 is 7.8-18.1 ng/mL.

Species

Rhesus Macaque

Source

E. coli

Tag

Tag Free

Accession

F6S9W7 (V114-G281)

Gene ID
Molecular Construction
N-term
TRAIL (V114-G281)
Accession # F6S9W7
C-term
Synonyms
Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand; Apo-2L; TNF-Related Apoptosis-Inducing Ligand; Protein TRAIL; CD253; TNFSF10; APO2L; TRAIL
Molecular Weight

Approximately 19.6 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

TRAIL/TNFSF10 Protein, Rhesus Macaque Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TRAIL/TNFSF10 Protein, Rhesus Macaque
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