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TRAIL/TNFSF10 Protein, Rhesus Macaque

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고객리뷰

Based on 1 publication(s) in Google Scholar

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein mainly interacts with TRAIL-R1 and TRAIL-R2, and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. TRAIL Protein can recruit FADD and further recruit and activates caspase-8 after binding to DR4 or DR5. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell. TRAIL/TNFSF10 Protein, Rhesus Macaque is a recombinant Rhesus Macaque TRAIL (V114-G281) without any tag, which is expressed in E. coli.

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  • Species: Rhesus Macaque
  • Source: E. coli
  • 보관:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • TRAIL/TNFSF10 Protein, Rhesus Macaque Product Image
  • TRAIL/TNFSF10 Protein, Rhesus Macaque Product Image
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  • Biological Activity
  • Technical Parameters
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Biological Activity

제품 설명

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein mainly interacts with TRAIL-R1 and TRAIL-R2, and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. TRAIL Protein can recruit FADD and further recruit and activates caspase-8 after binding to DR4 or DR5. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways[1][2]. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell[1]. TRAIL/TNFSF10 Protein, Rhesus Macaque is a recombinant Rhesus Macaque TRAIL (V114-G281) without any tag, which is expressed in E. coli.

Background

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein is expressed in various tissues, especially in the spleen, lung, and prostate. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell. TRAIL proteins on NK and T cells is critical for controlling virus infections and tumor immune surveillance[1][2].
Human TRAIL consists of cytoplasmic domain (M1-V17), helical domain (L18-F38), and extracellular domain (T39-G281). Human TRAIL Protein shares < 70% common aa identity with mouse and rat. Mouse TRAIL Protein shares 86.94% common aa identity with rat.
TRAIL Protein mainly interacts with two agonistic TRAIL receptors (TRAIL-R1 and TRAIL-R2) and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. When binding to DR4 or DR5, TRAIL Protein can recruit FADD and further recruit and activates caspase-8. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways, such as NF-κB, PI3K/Akt, and MAPK pathway[1][2].
TRAIL induces apoptosis of tumor cells in a p53 independent manner. TRAIL-based therapies has high anti-tumor potential[3].

In Vitro

TRAIL (human, 1 ng/mL-1 μg/mL, 24 h) reduces cell viability of HepG2 cells[4].
TRAIL (human, 1-1 ng, 24 h) induces pathognomic features of asthma and stimulates the production of CCL2 in primary human bronchial epithelium cells[5].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized rhesus macaque TNFSF10 at 10 μg/mL (100 μL/well) can bind rhesus macaque TNFRSF10D-Fc and the EC50 is 7.8-18.1 ng/mL.

Technical Parameters

  • Species Rhesus Macaque
  • Source E. coli
  • Tag Tag Free
  • Accession

    F6S9W7 (V114-G281)

  • Gene ID
    694451  [NCBI]
  • Molecular Construction
    • N-term
    • TRAIL (V114-G281)
      Accession # F6S9W7
    • C-term
  • Protein Length

    Partial

  • Synonyms

    Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand; Apo-2L; TNF-Related Apoptosis-Inducing Ligand; Protein TRAIL; CD253; TNFSF10; APO2L; TRAIL

  • AA Sequence

    VRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIQEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGLFELKKDDRIFVSVTNEHLIDMDHEASFFGAFLVG

  • Predicted Molecular Mass

    19.6 kDa

  • 분자량

    Approximately 19 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

선적

Room temperature in continental US; may vary elsewhere.

References

[1]. Cardoso Alves L, et al. The multifaceted role of TRAIL signaling in cancer and immunity. FEBS J. 2021 Oct;288(19):5530-5554. [Content Brief]

[2]. Zhong HH, et al. TRAIL-based gene delivery and therapeutic strategies. Acta Pharmacol Sin. 2019 Nov;40(11):1373-1385. [Content Brief]

[3]. Snajdauf M, et al. The TRAIL in the Treatment of Human Cancer: An Update on Clinical Trials. Front Mol Biosci. 2021 Mar 10;8:628332. [Content Brief]

[4]. Ganten TM, et al. Preclinical differentiation between apparently safe and potentially hepatotoxic applications of TRAIL either alone or in combination with chemotherapeutic drugs. Clin Cancer Res. 2006 Apr 15;12(8):2640-6. [Content Brief]

[5]. Weckmann M, et al. Critical link between TRAIL and CCL20 for the activation of TH2 cells and the expression of allergic airway disease. Nat Med. 2007 Nov;13(11):1308-15. [Content Brief]

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Calculators

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

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TRAIL/TNFSF10 Protein, Rhesus Macaque Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • TRAIL/TNFSF10 Protein, Rhesus Macaque Product Image