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Cat. No.: HY-174827

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POLRMT-IN-2

POLRMT-IN-2 is a potent POLRMT inhibitor. POLRMT-IN-2 exhibits strong antiproliferative activity in MOLM-13 cells, with an IC₅₀ of 1.01 μM. POLRMT-IN-2 disrupts mitochondrial function and induces apoptosis in MOLM-13cells. POLRMT-IN-2 can be used for the study of acute myeloid leukemia(AML).

For research use only. We do not sell to patients.

Formula: C₃₄H₃₂N₄O₅
Molecular Weight:576.64

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

POLRMT-IN-2 Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

POLRMT-IN-2 (Compound 10a) (0-100 μM, 48 h) inhibits POLRMT activity dose-dependently in MOLM-13 cells, with an EC₅₀ value of 2.208 μΜ for ND1 expression suppression^[1].

POLRMT-IN-2 (48 h) shows anti-proliferation activity in cells with IC₅₀s of 1.01 μΜ (MOLM-13 cells), 3.24 μΜ (MV4-11 cells) and 14.17 μΜ (HL-60 cells)^[1].

POLRMT-IN-2 (5 μM, 48 h) can inhibit the proliferation of MOLM-13 cells, as evidenced by the reduction in EdU-positive cells and decreased Ki67 expression^[1].

POLRMT-IN-2 (0-10 μM, 48 h) disrupts mitochondrial function in MOLM-13 cells, as demonstrated by dose-dependent increases in ROS levels, decreases in ATP content, and reduction of mitochondrial membrane potential^[1].

POLRMT-IN-2 (0-10 μM, 48 h) induces apoptosis and reduces the expression of both AKT and p-AKTSer473 in MOLM-13 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[1]

Cell Line:

MOLM 13 cells
Concentration:

5 μM
Incubation Time:

48 h
Result:

Increased the positive areas of tunel staining.

Western Blot Analysis^[1]

Cell Line:

MOLM 13 cells
Concentration:

0, 2.5, 5, 10 μM
Incubation Time:

48 h
Result:

Increased the expression of apoptosis marker Bax significantly.
Decreased the expression of Bcl-2 was significantly.

Chemical Information

Molecular Weight 576.64
Formula C₃₄H₃₂N₄O₅
SMILES

CC1=CC=C(C(C)=C1)C2=CC(OC3=C2C=CC(O[C@@H](C(N4C5CN(C(NC6=CC=CC(C#N)=C6)=O)CC4CC5)=O)C)=C3)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liu T, Cheng X, Wang Y, Hao W, Wang H, Zhang K, Wang J. Design, Synthesis and Biological Evaluation of POLRMT Inhibitors for the Treatment of Acute Myeloid Leukemia. Chem Biol Drug Des. 2025 May;105(5):e70127. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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