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Biotin TSA（200×）

(Synonyms: Biotin Tyramide)
8 Cited Publications
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Based on 8 publication(s) in Google Scholar

Biotin TSA (200×) (Biotin Tyramide) is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification.

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  • Biotin TSA（200×） Chemical Structure
    Biotin TSA（200×） Chemical Structure
  • Experimental Validation Results for Biotin TSA（200×）
  • Experimental Validation Results for Biotin TSA（200×）
  • Experimental Validation Results for Biotin TSA（200×）
  • Experimental Validation Results for Biotin TSA（200×）
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  • Biotin TSA（200×） Chemical Structure
    Biotin TSA（200×） Chemical Structure
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    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Nature. 2025 May;641(8061):202-210. [Abstract]

    Customer Validation
     HEK293T cells expressing STING-APEX2 were first treated with 0.5 μM diBAZI, OVA peptide, or ABM5-OVA for 2 h, then with Biotinylated tyramide (500 μM; 30 min) and H2O2. Biotinylated proteins were pulled down by streptavidin magnetic beads and analyzed by western blot for TAP1 and proteasomes.

  • 1/16

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]

    Customer Validation
     Biotinyl tyramide (500 μmol/L; 30 min). Immunofluorescence-validated APEX2-MST1 labeling specificity in KYSE150 cells, biotin red, MST1 green; right: expression of the APEX2-MST1 fusion protein was observed.

  • 1/16

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]

    Customer Validation
     Biotinyl tyramide (500 μmol/L; 30 min) was used. Streptavidin-HRP Western blotting was performed to detect induced protein biotinylation in lysates from cells expressing APEX2-MST1.

  • 1/16

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Apr 25;14(1):2378. [Abstract]

    Customer Validation
     Proximity labelling of peroxidase-based approach. Biotin-phenol (Biotinyl tyramide, 500 μM; 30 min) was oxidized into reactive phenoxyl radicals with hydrogen peroxide existence, thus enabling the proximal labelling. Biotinylated proteins from a proximity labeling experiment analyzed by biotin and streptavidin-HRP blot in E11.5 wild-type kidney rudiments. Clear changes in band pattern were observed for HRP-Flag-Ism1 treated samples compared with ligand-free samples. WCL, whole cell lysis.

  • Biotin TSA（200×）