Biotinyl tyramide

Cat. No.: HY-125658 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Biotinyl tyramide is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols.

For research use only. We do not sell to patients.

Biotinyl tyramide Chemical Structure

CAS No. : 41994-02-9

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

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Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Biotinyl tyramide

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Purity & Documentation
References
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Description

Biotinyl tyramide is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols^[1]^[2]^[3]^[4].

Molecular Weight

363.47

Appearance

Solid

Formula

C₁₈H₂₅N₃O₃S

CAS No.

41994-02-9

SMILES

O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (275.13 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7513 mL	13.7563 mL	27.5126 mL
5 mM	0.5503 mL	2.7513 mL	5.5025 mL
10 mM	0.2751 mL	1.3756 mL	2.7513 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (245 KB) HNMR (255 KB) LCMS (257 KB)

Handling Instructions (2659 KB)

References

[1]. Mark F Evans, et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens. BMC Clin Pathol. 2003 Jun 11;3(1):2. [Content Brief]

[2]. Bobrow MN, et al. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J Immunol Methods. 1989 Dec 20;125(1-2):279-85. [Content Brief]

[3]. Kim SH, et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining. J Histochem Cytochem. 2003 Jan;51(1):129-32. [Content Brief]

[4]. Evans MF , et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens. BMC Clin Pathol. 2003 Jun 11;3(1):2.

[5]. Hunyady B, et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining. J Histochem Cytochem. 1996 Dec;44(12):1353-62. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

Biotinyl tyramide41994-02-9OthersTSAInhibitorinhibitorinhibit

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Biotinyl tyramide

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