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  4. Biotin TSA（200×）

Biotin TSA (200×) (Biotin Tyramide) is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification.

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Biotin TSA（200×） Related Antibodies

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    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Nature. 2025 May;641(8061):202-210.  [Abstract]

    HEK293T cells expressing STING-APEX2 were first treated with 0.5 μM diBAZI, OVA peptide, or ABM5-OVA for 2 h, then with Biotinylated tyramide (500 μM; 30 min) and H2O2. Biotinylated proteins were pulled down by streptavidin magnetic beads and analyzed by western blot for TAP1 and proteasomes.

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543.

    Biotinyl tyramide (500 μmol/L; 30 min). Immunofluorescence-validated APEX2-MST1 labeling specificity in KYSE150 cells, biotin red, MST1 green; right: expression of the APEX2-MST1 fusion protein was observed.

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543.

    Biotinyl tyramide (500 μmol/L; 30 min) was used. Streptavidin-HRP Western blotting was performed to detect induced protein biotinylation in lysates from cells expressing APEX2-MST1.

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Apr 25;14(1):2378.

    Biotinyl tyramide (5 μM; 30 min). Biotinylated proteins from a proximity labeling experiment were analyzed by biotin and streptavidin HRP blot in E11.5 wild-type kidney rudiments. Clear changes in band pattern were observed for HRP-Flag-Ism1 treated samples compared with ligand-free samples.

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Apr 25;14(1):2378.

    Biotinyl tyramide (5 μM; 30 min). Western blotting stained for Ism1 indicated that the HRP-conjugated Flag-Ism1 was detected, and the efficiency of HRP conjugation was approximately 70% (the size of the HPR-conjugated protein shifted from 70 kDa to 110 kDa and even over 170 kDa).

    Biotin TSA（200×） purchased from MedChemExpress. Usage Cited in: Genome Biol. 2022 Dec 15;23(1):259.  [Abstract]

    Biotinyl tyramide (3 mM; 3 min). Profiling of hemin binding sites in cells using G4 self-biotinylation reaction and CUT&Tag. UCSC genome browser snapshots of G4 self-biotinylation signals in HEK293T cells in the presence or absence of 80 μM TMPyP4 or PDS competitors were obtained.

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    Description

    Biotin TSA (200×) (Biotin Tyramide) is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification[1][2][3][4][5].

    In Vitro

    Biotinyl tyramide (100 μM, 5min) and hemin (HY-19424) enhance biotinylation of starved U2OS cells after validating the G4-specific biotinylation activity of RNA-hemin complexes in vitro[6].
    Biotinyl tyramide (27.5 μM, 5min) binds to the tyrosine side chains of cell surface proteins in HaCaT wild-type cells or dual oxidase 1 (DUOX1) knockout cells[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Biotin TSA（200×） Related Antibodies
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    [Biotin TSA（200×）]
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Biotin TSA（200×）Biotin TyramideFluorescent Dyehigh-densityhorseradish peroxidaseHRPELISAIFIHCInhibitorinhibitorinhibit

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