Tris Buffered Saline

Tris Buffered Saline is a commonly used buffered salt for molecular biology experiments, which is composed of trimethylmethacrylate (Tris) and potash (NaCl). It has special features such as adjusting pH value and ionic strength, high solubility and low toxicity, and is widely used in experiments such as DNA/RNA extraction, PCR, and protein electrophoresis.
In DNA/RNA extraction experiments, Tris Buffered Saline is usually used for DNA/RNA purification, accelerating the reaction process and balancing to determine the DNA/RNA structure. Tris Buffered Saline can effectively protect DNA/RNA from degradation by deoxyribonuclease (DNase) and ribonuclease (RNase).
In PCR experiments. Tris Buffered Saline can be used in the preparation of PCR reaction systems.
Tris Buffered Saline can also be used as a gel rinse to maintain gel stability and pH of the electrophoresis solution.
MCE offers Tris Buffered Saline in tablet form. One tablet of Tris Buffered Saline can be dissolved in 15 mL of deionized water to yield 0.05M Tris and 0.15M sodium chloride, pH 7.6 at 25°C.

Tris Buffered Saline can be used in the following studies:
Immunogold-silver staining (IGSS) method to study immunoglobulins in reactive human tonsils^[1].
Studying aggregation of purified samples of α-synuclein^[2].
As a buffer for Western blot analysis^[3].
Time-resolved fluorescence immunoassay^[4].
As a buffer for light microscopy immunohistochemistry^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.