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Art. -Nr.: HY-P2893

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Creatinase, Actinobacteria

(Synonyms: Creatine amidohydrolase)

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Creatinase, Actinobacteria (Creatine amidohydrolase) is a hydrolase that catalyzes the hydrolysis of creatine into sarcosine and urea, and plays an important role in the enzymatic determination of creatinine concentration.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 37340-58-2

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Creatinase, Actinobacteria Chemical Structure

CAS. Nr.: 37340-58-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

Product Information
This product can hydrolyze creatine and is used in the development and preparation of enzymatic creatinine reagents.
Molecular weight: 48 kDa (SDS-PAGE)
Isoelectric point: 4.6
Inhibitors: Ag ⁺, Cu ²⁺, Hg ²⁺
Optimum: pH 7.5
Optimum temperature: 45-50°C
pH stability 4.5-10.0 (25°C, 16 h)

Instructions
Can be dissolved in water (1 mg/mL), or according to specific experimental references (such as using 50 mM PBS, pH 7.5).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

3.5.3.3

Enzyme Activity

≥10 U/mg soild

Unit Definition

One unit is defined as the amount of enzyme that can react creatine to produce 1 μmol sarcosine per minute at 37°C and pH 5.5.

Chemical Information

CAS. Nr. 37340-58-2
Appearance Solid
Color White to off-white
SMILES

[Creatinase, Actinobacteria]
Synonyms

Creatine amidohydrolase
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 100 mg/mL (Need ultrasonic)

View the Complete Stock Solution Preparation Table

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (264 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. J Schumann, et al. Stabilization of creatinase from Pseudomonas putida by random mutagenesis. Protein Sci. 1993 Oct;2(10):1612-20. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biologische Aktivität
- Beschreibung
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise

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Creatinase, Actinobacteria

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen