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Cat. No.: HY-P2893

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Creatinase, Actinobacteria

(Synonyms: Creatine amidohydrolase)

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Creatinase, Actinobacteria (Creatine amidohydrolase) is a hydrolase that catalyzes the hydrolysis of creatine into sarcosine and urea, and plays an important role in the enzymatic determination of creatinine concentration.

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CAS No.: 37340-58-2

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Creatinase, Actinobacteria Chemical Structure

CAS No.: 37340-58-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Solvant et solubilité
- In Vitro
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Voir tous les produits spécifiques à Isoform Endogenous Metabolite :

In Vitro

Product Information
This product can hydrolyze creatine and is used in the development and preparation of enzymatic creatinine reagents.
Molecular weight: 48 kDa (SDS-PAGE)
Isoelectric point: 4.6
Inhibitors: Ag ⁺, Cu ²⁺, Hg ²⁺
Optimum: pH 7.5
Optimum temperature: 45-50°C
pH stability 4.5-10.0 (25°C, 16 h)

Instructions
Can be dissolved in water (1 mg/mL), or according to specific experimental references (such as using 50 mM PBS, pH 7.5).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

3.5.3.3

Enzyme Activity

≥10 U/mg soild

Unit Definition

One unit is defined as the amount of enzyme that can react creatine to produce 1 μmol sarcosine per minute at 37°C and pH 5.5.

Chemical Information

CAS No. 37340-58-2
Appearance Solid
Color White to off-white
SMILES

[Creatinase, Actinobacteria]
Synonyms

Creatine amidohydrolase
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvant et solubilité

In Vitro:

H₂O : 100 mg/mL (Need ultrasonic)

View the Complete Stock Solution Preparation Table

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (264 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. J Schumann, et al. Stabilization of creatinase from Pseudomonas putida by random mutagenesis. Protein Sci. 1993 Oct;2(10):1612-20. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Activité biologique
- Description
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Solvant et solubilité
- In Vitro
Pureté et documentation
Références

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Creatinase, Actinobacteria

Voir tous les produits spécifiques à Isoform Endogenous Metabolite