3'-BPANeuGc
3'-BPANeuGc is a high-affinity selective Siglec-G ligand. Lipid nanoparticles surface-modified with antigens and 3'-BPANeuGc inhibit BCR signal transduction. 3'-BPANeuGc is applicable to antigen-specific tolerance research.
For research use only. We do not sell to patients.
- CAS No.: 1596134-81-4
- Formula: C41H58N4O20
- Molecular Weight:926.91
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
3'-BPANeuGc (1-4%; 40 min at 4°C) is a selective, high-affinity ligand for Siglec-G, as demonstrated by specific binding to Siglec-G-expressing BW5147 cells and murine splenic B cells, with no cross-reactivity to other murine siglecs[1].
3'-BPANeuGc (1-4%) when co-displayed with anti-IgM on liposomes substantially suppresses calcium flux in both murine peritoneal B1a cells and splenic B2 cells in a Siglec-G-dependent manner[1].
3'-BPANeuGc (1-4%; 3 min) when co-displayed with HEL on liposomes-mediated recruitment of Siglec-G to the BCR strongly dampens proximal and distal BCR signaling cascade components in splenic HEL-reactive MD4 B cells in an Shp-1-dependent manner[1].
3'-BPANeuGc (1-4%; 4 days) when co-displayed with HEL on liposomes inhibits proliferation and reduces survival of HEL-reactive MD4 B cells in a Siglec-G-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:splenic HEL-reactive MD4 B cells (wild-type and Shp-1-deficient)
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Concentration:1-4% (in 5 μM liposomes containing HEL)
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Incubation Time:3 min
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Result:Suppressed phosphorylation or degradation of multiple BCR signaling components, including those involved in MAP kinase, Erk, NFkB, and Akt pathways, when co-displayed with HEL on liposomes, compared to liposomes displaying HEL alone.
Showed no inhibitory effect in Shp-1-deficient (Moth eaten) B cells.
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Cell Line:HEL-reactive MD4 B cells (wild-type and Siglec-G-deficient)
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Concentration:1-4% (in liposomes containing HEL)
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Incubation Time:4 days
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Result:Caused pronounced diminishment in proliferation and a significant reduction in live cell numbers when co-displayed with HEL on liposomes, compared to liposomes displaying HEL alone.
Showed no such effects in Siglec-G-deficient MD4 B cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6J (wild-type; Siglec-G-deficient; MD4 transgenic, IgMHEL-expressing)[1]
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Dosage:0.5 mM total lipid concentration (delivered in 200 µl PBS)
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Administration:i.p.; single dose; i.v.; single dose
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Result:Showed greatly reduced anti-NP IgM and IgG titers compared to mice receiving NP-only liposomes.
Failed to produce anti-NP antibodies upon subsequent challenge with NP-only liposomes.
Chemical Information
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CAS No. 1596134-81-4
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Molecular Weight 926.91
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Formula C41H58N4O20
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SMILES
OC([C@@]1(O[C@@]([C@@H]([C@H](C1)O)NC(CO)=O)([H])[C@H](O)[C@H](O)CNC(CC2=CC=C(C3=CC=CC=C3)C=C2)=O)O[C@@H]4[C@H]([C@@H](O[C@@H]([C@@H]4O)CO)O[C@@H]5[C@H](O[C@H]([C@@H]([C@H]5O)NC(C)=O)OCCN)CO)O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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