DM-Nitrophen
Based on 1 Customer Validation
DM-Nitrophen is a photolabile caged calcium compound that acts as a calcium releaser. DM-Nitrophen binds Ca2+ with high affinity and Mg2+ with considerable affinity before photolysis. DM-Nitrophen releases Ca2+ into the cytosol upon ultraviolet light irradiation. DM-Nitrophen induces calcium concentration pulses, triggers cytosolic calcium transients, promotes calcium-dependent exocytosis. DM-Nitrophen can be used for the research of calcium-dependent cellular processes.
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- Pureza: 98.40%
- No. CAS: 117367-86-9
- Fòrmula: C18H23N3O12
- Peso molecular:473.39
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
DM-nitrophen forms biphasically decaying aci-nitro intermediates and releases calcium ions efficiently upon laser photolysis, with calcium release rate being pH-dependent (minimum at physiological pH) and capable of rapidly generating transient free calcium pulses that quickly reach a steady state[1].
DM-nitrophen induces obvious intracellular calcium elevation and promotes exocytosis after UV flash photolysis in cultured rat pituitary melanotrophs, and barely affects the calcium dissociation constant of Fura-2[2].
DM-Nitrophen activates typical transient Ca2+-dependent Cl- currents consistent with voltage-triggered counterparts in rat DRG neurons after flash photolysis, and can reactivate such currents during decay phase to confirm current deactivation instead of inactivation[3].
DM-Nitrophen only elicits weak current responses under depolarized holding potentials and exerts no influence on the inactivation process of voltage-gated Ca2+ currents in target neurons[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 117367-86-9
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Appearance Solid
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Peso molecular 473.39
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Fòrmula C18H23N3O12
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Color Light yellow to yellow
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SMILES
O=[N+](C1=CC(OC)=C(OC)C=C1C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O)[O-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
H2O : 25 mg/mL (52.81 mM; Need ultrasonic)
DMSO : 1 mg/mL (2.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Ellis-Davies GC, et al. Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen. Biophys J. 1996;70(2):1006-1016. [Content Brief]
[2]. Parsons TD, et al. Millisecond studies of calcium-dependent exocytosis in pituitary melanotrophs: comparison of the photolabile calcium chelators nitrophenyl-EGTA and DM-nitrophen. Cell Calcium. 1996;19(3):185-192. [Content Brief]
[3]. Currie KP, et al. Activation of Ca(2+)-dependent Cl- currents in cultured rat sensory neurones by flash photolysis of DM-nitrophen. J Physiol. 1995;482 ( Pt 2)(Pt 2):291-307. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.1124 mL | 10.5621 mL | 21.1242 mL | 52.8106 mL |
| H2O | 5 mM | 0.4225 mL | 2.1124 mL | 4.2248 mL | 10.5621 mL |
| 10 mM | 0.2112 mL | 1.0562 mL | 2.1124 mL | 5.2811 mL | |
| 15 mM | 0.1408 mL | 0.7041 mL | 1.4083 mL | 3.5207 mL | |
| 20 mM | 0.1056 mL | 0.5281 mL | 1.0562 mL | 2.6405 mL | |
| 25 mM | 0.0845 mL | 0.4225 mL | 0.8450 mL | 2.1124 mL | |
| 30 mM | 0.0704 mL | 0.3521 mL | 0.7041 mL | 1.7604 mL | |
| 40 mM | 0.0528 mL | 0.2641 mL | 0.5281 mL | 1.3203 mL | |
| 50 mM | 0.0422 mL | 0.2112 mL | 0.4225 mL | 1.0562 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DM-Nitrophen Related Classifications
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