DM-Nitrophen tertasodium 

DM-Nitrophen tertasodium is a photolabile caged calcium compound that acts as a calcium releaser. DM-Nitrophen tertasodium binds Ca²⁺ with high affinity and Mg²⁺ with considerable affinity before photolysis. DM-Nitrophen tertasodium releases Ca²⁺ into the cytosol upon ultraviolet light irradiation. DM-Nitrophen tertasodium induces calcium concentration pulses, triggers cytosolic calcium transients, promotes calcium-dependent exocytosis. DM-Nitrophen tertasodium can be used for the research of calcium-dependent cellular processes^[1][2][3].

DM-Nitrophen tertasodium forms biphasically decaying aci-nitro intermediates and releases calcium ions efficiently upon laser photolysis, with calcium release rate being pH-dependent (minimum at physiological pH) and capable of rapidly generating transient free calcium pulses that quickly reach a steady state^[1].
DM-Nitrophen tertasodium induces obvious intracellular calcium elevation and promotes exocytosis after UV flash photolysis in cultured rat pituitary melanotrophs, and barely affects the calcium dissociation constant of Fura-2^[2].
DM-Nitrophen tertasodium activates typical transient Ca²⁺-dependent Cl^- currents consistent with voltage-triggered counterparts in rat DRG neurons after flash photolysis, and can reactivate such currents during decay phase to confirm current deactivation instead of inactivation^[3].
DM-Nitrophen tertasodium only elicits weak current responses under depolarized holding potentials and exerts no influence on the inactivation process of voltage-gated Ca²⁺ currents in target neurons^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

561.32

C₁₈H₁₉N₃Na₄O₁₂

291517-40-3

Solid

Light yellow to brown

O=C(CN(CC(N(CC(O[Na])=O)CC(O[Na])=O)C1=CC(OC)=C(C=C1[N+]([O-])=O)OC)CC(O[Na])=O)O[Na]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Ellis-Davies GC, et al. Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen. Biophys J. 1996 Feb;70(2):1006-16.  [Content Brief]

  [2]. Parsons TD, et al. Millisecond studies of calcium-dependent exocytosis in pituitary melanotrophs: comparison of the photolabile calcium chelators nitrophenyl-EGTA and DM-nitrophen. Cell Calcium. 1996;19(3):185-192.  [Content Brief]

  [3]. Currie KP, et al. Activation of Ca(2+)-dependent Cl- currents in cultured rat sensory neurones by flash photolysis of DM-nitrophen. J Physiol. 1995;482 ( Pt 2)(Pt 2):291-307.