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  4. DM-Nitrophen

DM-Nitrophen is a photolabile caged calcium compound that acts as a calcium releaser. DM-Nitrophen binds Ca2+ with high affinity and Mg2+ with considerable affinity before photolysis. DM-Nitrophen releases Ca2+ into the cytosol upon ultraviolet light irradiation. DM-Nitrophen induces calcium concentration pulses, triggers cytosolic calcium transients, promotes calcium-dependent exocytosis. DM-Nitrophen can be used for the research of calcium-dependent cellular processes.

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DM-Nitrophen

DM-Nitrophen Chemical Structure

CAS No. : 117367-86-9

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DM-Nitrophen Related Antibodies

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Description

DM-Nitrophen is a photolabile caged calcium compound that acts as a calcium releaser. DM-Nitrophen binds Ca2+ with high affinity and Mg2+ with considerable affinity before photolysis. DM-Nitrophen releases Ca2+ into the cytosol upon ultraviolet light irradiation. DM-Nitrophen induces calcium concentration pulses, triggers cytosolic calcium transients, promotes calcium-dependent exocytosis. DM-Nitrophen can be used for the research of calcium-dependent cellular processes[1][2][3].

In Vitro

DM-nitrophen forms biphasically decaying aci-nitro intermediates and releases calcium ions efficiently upon laser photolysis, with calcium release rate being pH-dependent (minimum at physiological pH) and capable of rapidly generating transient free calcium pulses that quickly reach a steady state[1].
DM-nitrophen induces obvious intracellular calcium elevation and promotes exocytosis after UV flash photolysis in cultured rat pituitary melanotrophs, and barely affects the calcium dissociation constant of Fura-2[2].
DM-Nitrophen activates typical transient Ca2+-dependent Cl- currents consistent with voltage-triggered counterparts in rat DRG neurons after flash photolysis, and can reactivate such currents during decay phase to confirm current deactivation instead of inactivation[3].
DM-Nitrophen only elicits weak current responses under depolarized holding potentials and exerts no influence on the inactivation process of voltage-gated Ca2+ currents in target neurons[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DM-Nitrophen Related Antibodies
Molecular Weight

473.39

Formula

C18H23N3O12
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+](C1=CC(OC)=C(OC)C=C1C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (52.81 mM; Need ultrasonic)

DMSO : 1 mg/mL (2.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.1124 mL 10.5621 mL 21.1242 mL 52.8106 mL
H2O 5 mM 0.4225 mL 2.1124 mL 4.2248 mL 10.5621 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL 5.2811 mL
15 mM 0.1408 mL 0.7041 mL 1.4083 mL 3.5207 mL
20 mM 0.1056 mL 0.5281 mL 1.0562 mL 2.6405 mL
25 mM 0.0845 mL 0.4225 mL 0.8450 mL 2.1124 mL
30 mM 0.0704 mL 0.3521 mL 0.7041 mL 1.7604 mL
40 mM 0.0528 mL 0.2641 mL 0.5281 mL 1.3203 mL
50 mM 0.0422 mL 0.2112 mL 0.4225 mL 1.0562 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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DM-Nitrophen Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DM-Nitrophen117367-86-9Biochemical Assay Reagentsmagnesiumcalcium ioncalcium-dependent exocytosisrat pituitary melanotrophsaci-nitro intermediatescytosolic calcium transientsCa2+-activated Cl-currentsvoltage-gated Ca2+ currentscytosolrat DRG neuronesInhibitorinhibitorinhibit

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