1. Epigenetics
    PI3K/Akt/mTOR
  2. AMPK
  3. IM156

IM156 (Synonyms: HL156A; HL271 acetate)

Cat. No.: HY-136093A Purity: 99.80%
Handling Instructions

IM156 (HL156A; HL271 acetate), a chemical derivative of Metformin (HY-B0627), is a potent and orally active AMPK activator that increases AMPK phosphorylation. IM156 attenuates aging-associated cognitive impairment in animal model. IM156 is a potent oxidative phosphorylation (OXPHOS) inhibitor which can be used for the research of solid tumors.

For research use only. We do not sell to patients.

IM156 Chemical Structure

IM156 Chemical Structure

CAS No. : 1422365-94-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 330 In-stock
Estimated Time of Arrival: December 31
5 mg USD 300 In-stock
Estimated Time of Arrival: December 31
10 mg USD 480 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1350 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1950 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

IM156 (HL156A; HL271 acetate), a chemical derivative of Metformin (HY-B0627), is a potent and orally active AMPK activator that increases AMPK phosphorylation. IM156 attenuates aging-associated cognitive impairment in animal model[1][2]. IM156 is a potent oxidative phosphorylation (OXPHOS) inhibitor which can be used for the research of solid tumors[3].

IC50 & Target

AMPK[1][2], OXPHOS[3]

In Vitro

IM156 (0.31-10 μM) phosphorylates AMPKα1 Thr172 in a dose- and time-dependent manner in NIH3T3 mouse fibroblast cells[1].
IM156 does not affect the expression of key factors involved in glucose homeostasis such as glucose-6-phosphatase (G6pase) or phosphoenolpyruvate carboxykinase 1 (Pck1)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: NIH3T3 cells
Concentration: 0.31 μM, 0.62 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM
Incubation Time: 4 hours
Result: Significantly increased the AMPK phosphorylation rate.
In Vivo

IM156 does not affect metabolic regulation assessed by body weight, blood glucose, insulin levels and lipid metabolite content in mice with diet-induced obesity[1].
IM156 (50 mg/kg; for 2 months) does not affect body weight, general locomotion, or anxiety[2].
IM156 significantly attenuates the aging-induced decline in novel object recognition memory and spatial working memory[2].
IM156 significantly increases AMPK activation in the hippocampus of aged mice[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J mice (young group/12-16 weeks, old groups/20-22 months)[2]
Dosage: 50 mg/kg
Administration: Oral administration (drinking water), for 2 months
Result: Attenuated age-related cognitive decline.
Clinical Trial
Molecular Weight

375.35

Formula

C₁₅H₂₀F₃N₅O₃

CAS No.

1422365-94-3

SMILES

N=C(N1CCCC1)NC(NC2=CC=C(OC(F)(F)F)C=C2)=N.CC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (266.42 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6642 mL 13.3209 mL 26.6418 mL
5 mM 0.5328 mL 2.6642 mL 5.3284 mL
10 mM 0.2664 mL 1.3321 mL 2.6642 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.66 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.66 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.66 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.80%

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Keywords:

IM156HL156AHL271IM 156IM-156HL 271HL-271AMPKAMP-activated protein kinasemetformindementiaADcognitivefunctionmemoryage-relateddeclineoxidativephosphorylationOXPHOSInhibitorinhibitorinhibit

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IM156
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