Cat. No.: HY-136093

Lixumistat hydrochloride

(Synonyms: HL271; IM156 hydrochloride; HL156A hydrochloride)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Lixumistat (IM156) hydrochloride is a potent and orally active AMPK activator and OXPHOS inhibitor. Lixumistat hydrochloride strongly activates AMPK, while it lacks the systemic metabolic regulatory effects of classic metformin, such as hypoglycemic and weight-lowering activities. Lixumistat hydrochloride exhibits significant therapeutic effects on cognitive decline associated with brain aging and pulmonary fibrosis.

For research use only. We do not sell to patients.

Purity: 99.25%
CAS No.: 1422365-52-3
Formula: C₁₃H₁₇ClF₃N₅O
Molecular Weight:351.76

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lixumistat hydrochloride Chemical Structure

CAS No.: 1422365-52-3

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

AMPK^[1]^[2], OXPHOS^[3]

All AMPK Isoforms :

In Vitro

Lixumistat (15 μM; 26 h) hydrochloride potently inhibits TGFβ1-induced mitochondrial oxidative phosphorylation reprogramming in WI-38 human embryonic lung fibroblasts, with an IC₅₀ of 14.7 μM, and does not alter TGFβ1-induced glycolytic changes^[2].
Lixumistat (7.5-30 μM; 26-48 h) hydrochloride inhibits TGFβ1-induced myofibroblast differentiation (α-SMA expression, IC₅₀ = 21.3 μM) and collagen deposition (IC₅₀ = 13.2 μM) in a dose-dependent manner in WI-38 human embryonic lung fibroblasts without reducing cell viability^[2].
Lixumistat (0.31-10 μM; 2-8 h) hydrochloride potently and rapidly induces the phosphorylation of AMPK at the Thr172 site in NIH3T3 mouse fibroblasts^[3].
Lixumistat (10 μM; 0-4 h) hydrochloride promotes the proteasome-dependent degradation of PER2 and CRY1 proteins in NIH3T3 mouse fibroblasts when co-incubated with Cycloheximide (HY-12320)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

NIH3T3 cells
Concentration:

0.31, 0.62, 1.25, 2.5, 5, 10 μM (dose-response); 10 μM (time-course)
Incubation Time:

4 h (dose-response); 2, 4, 8 h (time-course)
Result:

Dose-dependently stimulated AMPK phosphorylation, with significant increases observed at concentrations ≥ 1.25 μM after 4 h incubation.
Increased AMPK phosphorylation in a time-dependent manner, with significant elevations detected as early as 2 h after treatment with 10 μM.

In Vivo

Lixumistat (50 mg/kg; p.o.; administered daily for 2 months) hydrochloride significantly ameliorates age-related cognitive decline in aged C57BL/6J mice^[1].
Lixumistat (10-30 mg/kg; i.g.; once daily for 14 consecutive days) hydrochloride dose-dependently alleviates pulmonary fibrosis and inflammatory responses in male C57BL/6 mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J (male and female, 20~22 months of age)^[1]
Dosage:

50 mg/kg
Administration:

p.o.; daily; 2 months
Result:

Significantly improved age-related cognitive decline in aged C57BL/6J mice, with a 23.2% increase in novel object recognition discrimination ratio, 27.4% increase in spatial working memory %SAP, 47.3% increase in contextual fear memory freezing level, and 91.6% increase in hippocampal pAMPK relative intensity compared to controls

Animal Model:

C57BL/6 (male, 6-7 weeks of age, bleomycin-induced pulmonary fibrosis)^[2]
Dosage:

10 mg/kg; 30 mg/kg
Administration:

i.g.; daily; 14 days
Result:

Attenuated body weight loss, with significant improvements observed on study days 19 and 21.
Reduced bleomycin-induced increases in lung weight at both doses, with greater efficacy at 30 mg/kg.
Significantly reduced modified Ashcroft fibrosis scores.

Chemical Information

CAS No. 1422365-52-3
Appearance Solid
Molecular Weight 351.76
Formula C₁₃H₁₇ClF₃N₅O
Color White to off-white
SMILES

N=C(N1CCCC1)NC(NC2=CC=C(OC(F)(F)F)C=C2)=N.[H]Cl
Synonyms

HL271; IM156 hydrochloride; HL156A hydrochloride
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (1)

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Journal Impact Factor

Cell Death Discov

Long-term adaptation of lymphoma cell lines to hypoxia is mediated by diverse molecular mechanisms that are targetable with specific inhibitors. [Abstract]

2025 Feb 18;11(1):65. PMID: 39966387

Solvent & Solubility

In Vitro:

DMSO : 41.67 mg/mL (118.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8428 mL

14.2142 mL

28.4285 mL

5 mM

0.5686 mL

2.8428 mL

5.6857 mL

10 mM

0.2843 mL

1.4214 mL

2.8428 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bang E, et al. The Improving Effect of HL271, a Chemical Derivative of Metformin, a Popular Drug for Type II Diabetes Mellitus, on Aging-induced Cognitive Decline. Exp Neurobiol. 2018;27(1):45-56. [Content Brief]

[2]. Willette RN, et al. Modulation of Oxidative Phosphorylation with IM156 Attenuates Mitochondrial Metabolic Reprogramming and Inhibits Pulmonary Fibrosis. J Pharmacol Exp Ther. 2021;379(3):290-300. [Content Brief]

[3]. Row H, et al. HL271, a novel chemical compound derived from metformin, differs from metformin in its effects on the circadian clock and metabolism. Biochem Biophys Res Commun. 2016;469(3):783-789. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8428 mL	14.2142 mL	28.4285 mL	71.0712 mL
	5 mM	0.5686 mL	2.8428 mL	5.6857 mL	14.2142 mL
	10 mM	0.2843 mL	1.4214 mL	2.8428 mL	7.1071 mL
	15 mM	0.1895 mL	0.9476 mL	1.8952 mL	4.7381 mL
	20 mM	0.1421 mL	0.7107 mL	1.4214 mL	3.5536 mL
	25 mM	0.1137 mL	0.5686 mL	1.1371 mL	2.8428 mL
	30 mM	0.0948 mL	0.4738 mL	0.9476 mL	2.3690 mL
	40 mM	0.0711 mL	0.3554 mL	0.7107 mL	1.7768 mL
	50 mM	0.0569 mL	0.2843 mL	0.5686 mL	1.4214 mL
	60 mM	0.0474 mL	0.2369 mL	0.4738 mL	1.1845 mL
	80 mM	0.0355 mL	0.1777 mL	0.3554 mL	0.8884 mL
	100 mM	0.0284 mL	0.1421 mL	0.2843 mL	0.7107 mL

Lixumistat hydrochloride

1 Cited Publications

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Lixumistat hydrochloride

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Publications Citing Use of MedChemExpress (MCE) Lixumistat hydrochloride

All AMPK Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Lixumistat hydrochloride Related Classifications

HY-136093 Related Classifications