D-Biotinol
Based on 1 Customer Validation
D-Biotinol is an orally active alcohol analogue of D-Biotin (HY-B0511). D-Biotinol can replace D-Biotin to restore the biotin deficiency induced by egg whites in rats. D-Biotinol can be used for the study of biotin deficiency disorders.
For research use only. We do not sell to patients.
- Purity: 97.06%
- CAS No.: 53906-36-8
- Formula: C10H18N2O2S
- Molecular Weight:230.33
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
D-Biotinol cannot replace biotin for the nutrition of microorganisms (such as Lactobacillus arabinosus, Lactobacillus casei, and Saccharomyces cerevisiae), as these microorganisms are unable to convert it into active biotin[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Biotin deficiency model established in rats[1]
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Dosage:0.75 and 1.5 μg
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Administration:Oral administration (p.o.), three times a week for 4 weeks
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Result:Increased the weight of rats and alleviated the symptoms of deficiencies (such as dermatitis, hair loss).
Chemical Information
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CAS No. 53906-36-8
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Appearance Solid
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Molecular Weight 230.33
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Formula C10H18N2O2S
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Color White to off-white
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SMILES
OCCCCC[C@H]1[C@](N2)([H])[C@@](CS1)([H])NC2=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (434.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Methanol : 25 mg/mL (108.54 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Methanol / DMSO | 1 mM | 4.3416 mL | 21.7080 mL | 43.4160 mL | 108.5399 mL |
| 5 mM | 0.8683 mL | 4.3416 mL | 8.6832 mL | 21.7080 mL | |
| 10 mM | 0.4342 mL | 2.1708 mL | 4.3416 mL | 10.8540 mL | |
| 15 mM | 0.2894 mL | 1.4472 mL | 2.8944 mL | 7.2360 mL | |
| 20 mM | 0.2171 mL | 1.0854 mL | 2.1708 mL | 5.4270 mL | |
| 25 mM | 0.1737 mL | 0.8683 mL | 1.7366 mL | 4.3416 mL | |
| 30 mM | 0.1447 mL | 0.7236 mL | 1.4472 mL | 3.6180 mL | |
| 40 mM | 0.1085 mL | 0.5427 mL | 1.0854 mL | 2.7135 mL | |
| 50 mM | 0.0868 mL | 0.4342 mL | 0.8683 mL | 2.1708 mL | |
| 60 mM | 0.0724 mL | 0.3618 mL | 0.7236 mL | 1.8090 mL | |
| 80 mM | 0.0543 mL | 0.2713 mL | 0.5427 mL | 1.3567 mL | |
| 100 mM | 0.0434 mL | 0.2171 mL | 0.4342 mL | 1.0854 mL |
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