16:0 PEG2000 PE
DPPE-PEG2000 ammonium (16:0 PEG5000 PE) is a polymer-lipid conjugate and LipoParticle stabilizer with a PEG chain of 5,000 g/mol molecular weight attached to its polar head, and it can be internalized by biological membranes. DPPE-PEG2000 ammonium enables LipoParticle to maintain colloidal stability after 20-fold dilution in PBS or cell culture medium, and prevents aggregate formation during lyophilization and rehydration. DPPE-PEG2000 ammonium helps enhance the non-cytotoxic property of LipoParticle formulations against human osteoblasts. DPPE-PEG2000 ammonium serves as a PEG lipid functional end group for synthesizing liposomes (LPs), is used in the design of conjugated polymer nanoparticles, and applies to research related to bone and joint infections.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 474922-84-4
- 分子式: (C2H4O)nC39H76NO10P.H3N
- 分子量:2700 (Average)
-
保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
生物活性
DPPE-PEG2000 ammonium (2 mol./mol. total lipid) imparts colloidal stability to LipoParticles upon 20-fold dilution in PBS or cell culture medium and enables freeze-drying and rehydration without significant aggregation, while maintaining defined size, size distribution, and surface charge profiles[1].
DPPE-PEG2000 ammonium (2 mol./mol. total lipid; 19.5-1250 μg/mL LipoParticles; 24 h) forms LipoParticles that are non-cytotoxic to human osteoblast MG63 cells at concentrations up to 1250 μg/mL after 24 h of incubation, with no reduction in cell metabolic activity[1].
DPPE-PEG2000 ammonium (2 mol./mol. total lipid; 3,000 colloids per cell; 24 h) forms LipoParticles that are efficiently internalized into human osteoblast MG63 cells after 24 h of incubation at a dose of 3,000 colloids per cell, with localization in the cellular cytoplasm[1].
DPPE-PEG2000 ammonium (2 mol./mol. total lipid; 22,000 colloids per cell; 24 h) forms LipoParticles that are internalized into human osteoblast MG63 cells after 24 h of incubation at a dose of 22,000 colloids per cell, with measurable fluorescence signals from both the PLGA core and lipid coating, and maintain high cell viability (89.7%)[1].
DPPE-PEG2000 ammonium (2 mol./mol. total lipid; 22,000 colloids per infected cell; 24 h) forms LipoParticles that are internalized into Staphylococcus aureus-infected human osteoblast MG63 cells after 24 h of incubation at a dose of 22,000 colloids per cell, with localization near intracellular bacteria[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 474922-84-4
-
性状 Solid
-
分子量 2700 (Average)
-
分子式 (C2H4O)nC39H76NO10P.H3N
-
Color White to off-white
-
SMILES
O=C(NCCOP(O)(OC[C@@H](COC(CCCCCCCCCCCCCCC)=O)OC(CCCCCCCCCCCCCCC)=O)=O)OCCOC.N.[n]
-
別名
DPPE-PEG2000; 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium)
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
純度とドキュメンテーション
-
データシート (266 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
取扱説明書 (2659 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
- DPPE-PEG2000
- 474922-84-4
- 16:0 PEG2000 PE
- 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]
- Liposome
- PC
- stroke
- FBS
- lipid nanoparticles
- pegylated liposomes
- polyethylene glycol-modified phosphatidylethanolamine lipid
- nonspecific protein adsorption
- serum
- zeta potential
- cholesterol
- Inhibitor
- inhibitor
- inhibit